The Expression Changes of Myelin and Lymphocyte Protein (MAL) Following Optic Nerve Crush in Adult Rats Retinal Ganglion Cells

被引：0
作者：
Yongsheng Huang
Yue Xu
Qiaochu Cheng
Shanshan Yu
Yi Gao
Qinmeng Shu
Cheng Yang
Yuan Sun
Jiawei Wang
Fan Xu
Xiaoling Liang
机构：
[1] Sun Yat-sen University,State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center
[2] Eye and ENT Hospital of Fudan University,Department of Ophthalmology
[3] Guangdong General Hospital,Department of Ophthalmology
[4] Guangdong Academy of Medical Sciences,undefined
来源：
Journal of Molecular Neuroscience | 2014年 / 54卷
关键词：
Optic nerve crush; MAL; Retinal ganglion cells; Apoptosis; Rats;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Myelin and lymphocyte protein (MAL), a component of compact myelin, is highly expressed in oligodendrocytes and Schwann cells. It has been reported that MAL may play a vital role in the process of neuronal apoptosis following acute spinal cord injury. However, acquaintance regarding its distribution and possible function in the retina is limited. Therefore, in a rodent model of optic nerve crush (ONC), the dynamic changes of MAL in retina was detected. The expression of MAL was mainly located in the retinal ganglion cells (RGCs) and was increased strongly after ONC. The peak of MAL expression appeared on the third day. In addition, there was a concomitant upregulation of active-caspase-3, which also co-localized with MAL in RGCs. Moreover, co-localization of MAL with terminal deoxynucleotidyl transferase-mediated biotinylated-dUTP nick-end labeling (TUNEL) was detected in RGCs after ONC. Collectively, all these results suggested that the upregulation of MAL might play an important role in the pathophysiology of RGCs after ONC.
引用
收藏
页码：614 / 621
页数：7
相关论文
共 304 条
[1]  
Allcutt D(1984)A qualitative comparison of the reactions of retinal ganglion cell axons to optic nerve crush in neonatal and adult mice Brain Res 318 231-240
[2]  
Berry M(2001)The role of lipid rafts in signalling and membrane trafficking in T lymphocytes J Cell Sci 114 3957-3965
[3]  
Sievers J(1987)cDNA cloning and sequence of MAL, a hydrophobic protein associated with human T-cell differentiation Proc Natl Acad Sci U S A 84 1997-2001
[4]  
Alonso MA(2003)Mechanisms of caspase activation Curr Opin Cell Biol 15 725-731
[5]  
Millan J(2003)PARP-1, a determinant of cell survival in response to DNA damage Exp Hematol 31 446-454
[6]  
Alonso MA(2008)MAL promoter hypermethylation as a novel prognostic marker in gastric cancer Br J Cancer 99 1802-1807
[7]  
Weissman SM(2010)Epigenetic silencing of MAL, a putative tumor suppressor gene, can contribute to human epithelium cell carcinoma Mol Cancer 9 296-751
[8]  
Boatright KM(1996)Unusual aspects of inflammation in the nervous system: Wallerian degeneration Neurobiol Aging 17 745-439
[9]  
Salvesen GS(2000)Nogo-A is a myelin-associated neurite outgrowth inhibitor and an antigen for monoclonal antibody IN-1 Nature 403 434-3954
[10]  
Bouchard VJ(2000)Cataractogenic lens injury prevents traumatic ganglion cell death and promotes axonal regeneration both in vivo and in culture Invest Ophthalmol Vis Sci 41 3943-544
12345678910