HPLC with Diode-Array Detection for Determination of Leflunomide in Tablets

被引：0
|
作者：
D. S. Miron
C. Soldattelli
E. E. S. Schapoval
机构：
[1] Universidade Federal do Rio Grande do Sul,Programa de Pós
[2] Universidade do Rio Grande do Sul,graduação em Ciências Farmacêuticas, Faculdade de Farmácia
来源：
Chromatographia | 2006年 / 63卷
关键词：
Column liquid chromatography; Diode-array detection; Leflunomide;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
We have developed and validated a simple HPLC method for analysis of leflunomide in tablets. Method conditions were determined by assay of a photodegraded sample of leflunomide. Optimum chromatographic performance was obtained with a C18 column and acetonitrile-water as mobile phase. Comparison of spectra recorded with a diode-array detector during elution of the leflunomide peak enabled determination of method specificity. The method is highly sensitive (detection limit 10 ng mL−1) and robust to deliberate variation of the conditions (RSD of peak area < 2.0%). Precision and accuracy were adequate over the concentration range 10 to 100 µg mL−1. These results show the proposed method is suitable for its intended use.
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页码：283 / 287
页数：4
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