Molecular characterization of tomato leaf curl Uganda virus infecting tomato plants in Kenya

被引:0
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作者
Ringeera Harun Kimathi
Fariha Wilisiani
Takaaki Mashiko
Yutaro Neriya
Ateka Elijah Miinda
Hisashi Nishigawa
Tomohide Natsuaki
机构
[1] Utsunomiya University,School of Agriculture
[2] Tokyo University of Agriculture and Technology,Department of Biological Production Science, United Graduate School of Agricultural Science
[3] Faculty of Agriculture,College of Agriculture and Natural Resources
[4] Institute of Agriculture STIPER,undefined
[5] Jomo Kenyatta University of Agriculture and Technology,undefined
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Tomato; Kenya; Agroinoculation;
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摘要
In 2017 in Kenya, we collected leaves from tomato plants with leaf curling. The leaves were infected with a begomovirus that was most similar to tomato leaf curl Uganda virus (ToLCUV-[UG:Iga:05]). Neither DNA-B nor satellite DNAs were detected. Recombination analyses indicated the virus was a chimera of tomato leaf curl Madagascar virus (ToLCMGV-Ats[MG:Tol:01]) and ToLCUV-[UG:Iga:05] (minor and major parents, respectively). An infectious clone induced symptoms on tomato, Nicotiana benthamiana, N. tabacum, and African nightshade. This is the first report describing the molecular characterization of tomato leaf curl disease in Kenya. GenBank/ENA/DDBJ accession number for the ToLCUV-[KE:Kia:17] sequences: LC437667.
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页码:513 / 518
页数:5
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