Rapid in vitro propagation of Eclipta alba (L.) Hassk. through high frequency axillary shoot proliferation

被引：27

作者：

Kashif Husain M. ^{[1
]}

Anis M. ^{[1
]}

机构：

[1] Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University

来源：

Acta Physiologiae Plantarum | 2006年 / 28卷 / 4期

关键词：

Greenhouse evaluation; Medicinal plant; Micropropagation; Nodal explants;

D O I：

10.1007/s11738-006-0028-8

中图分类号：

学科分类号：

摘要：

An efficient protocol has been developed for rapid in vitro propagation of Eclipta alba L. (Asteraceae) through axillary bud multiplication. Murashige and Skoog (MS) medium supplemented with BA (10 μM) was found to be most effective in breaking bud dormancy. An average number of 23 ± 0.57 shoots per explant was recorded after 30 days. Culture of node segments on fresh medium with lower concentration of BA (2 μM) enhanced the multiplication rate. A maximum of 79 ± 1.90 mean number of shoots were obtained after three subcultures without any decline in multiplication rate. The regenerated microshoots showed the most efficient rooting on half strength MS medium augmented with 0.5 μM IBA. Plantlets went through a hardening phase prior to ex vitro transfer and established in earthen pots containing garden soil; survival of about 90 %. The established plants were uniform and exhibited morphological characters identical to mother plants.

引用

页码：325 / 330

页数：5

共 23 条

[1]

The Wealth of India: Raw Materials, 3, pp. 127-128, (2003)

[2]

Opportunities and problems for the production and development of medicinal plants in India, Industry Highlights, 21, pp. 29-34, (1998)

[3]

Borthakur M., Dutta K., Nath S.C., Singh R.S., Micropropagation of Eclipta alba and Eupatorium adenophorum using a single-step nodal cutting technique, Plant Cell Tiss. Org. Cult., 62, pp. 239-242, (2000)

[4]

Casado J.P., Navarro M.C., Utrilla M.P., Martinez A., Jimenez J., Micropropagation of Santolina canescens Lagasca and in vitro volatiles production by shoot explants, Plant Cell Tiss. Org. Cult., 69, pp. 147-153, (2002)

[5]

Dias M.C., Almeida R., Romano A., Rapid clonal multiplication of Lavandula viridis L Her through in vitro axillary shoot proliferation, Plant Cell Tiss. Organ Cult., 68, pp. 99-102, (2002)

[6]

Faisal M., Anis M., Rapid mass propagation of Tylophora indica Merill via leaf callus culture, Plant Cell Tiss. Org. Cult., 75, pp. 125-129, (2003)

[7]

Faisal M., Ahmad N., Anis M., Shoot multiplication in Rauvolfia tetraphylla L. using thidiazuron, Plant Cell Tiss. Org. Cult., 80, pp. 187-190, (2005)

[8]

Franca S.C., Bertani B.W., Pereira A.M.S., Antihepatotoxic agent in micropropagated plantlets of Eclipta alba, Plant Cell Tiss. Org. Culture, 40, pp. 297-299, (1995)

[9]

Fraternale D., Giamperi L., Ricci D., Rocchi M.B.L., Micropropagation of Bupleurum fruticosum: The effect of tricontanol, Plant Cell Tiss. Organ Cult., 69, pp. 135-140, (2002)

[10]

Komalavalli N., Rao M.V., In vitro micropropagation of Gymnema sylvestre - A multipurpose medicinal plant, Plant Cell Tiss. Organ Cult., 61, pp. 97-105, (2000)

← 1 2 3 →