Regulation of ERK Phosphorylation by Ethanol in Fetal Cortical Neurons

被引：0
作者：
Haviryaji S. G. Kalluri
Maharaj K. Ticku
机构：
[1] The University of Texas Health Science Center,Department of Pharmacology
来源：
Neurochemical Research | 2003年 / 28卷
关键词：
Ethanol; MAP kinase; NMDA; CaM kinase;
D O I：
暂无
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学科分类号：
摘要：
Using fetal cortical neurons and phospho-specific MAP kinase antibody, we investigated the modulation of MAP kinase pathway by ethanol. Our results show that acute ethanol inhibited, while chronic treatment increased, the phosphorylation of MAP kinase per se; likewise AP-5 (a competitive antagonist for NMDA receptors) also increased the basal phosphorylation of MAP kinase following chronic treatment for 5 days. However, chronic ethanol or AP-5 induced MAP kinase phosphorylation was inhibited by KN-62 (calcium calmodulin dependent kinase inhibitor), suggesting the possible involvement of CaM (Calcium calmodulin) kinase. Immunoblot analysis revealed an upregulation of CaM kinase content in chronic ethanol and AP-5 treated cells. These results indicate that acute ethanol may inhibit, while chronic ethanol treatment increases, the basal phosphorylation of MAP kinase per se.
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页码：765 / 769
页数：4
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共 112 条
  • [1] Lovinger D. M.(1989)Ethanol inhibits NMDA-activated ion channels in hippocampal neurons Science 243 1721-1724
  • [2] White G.(1999)An update on GABA Brain. Res. Rev. 29 196-217
  • [3] Weight F. F.(1997) receptors Annu. Rev. Neurosci. 20 157-184
  • [4] Mehta A. K.(1997)Molecular genetic analysis of synaptic plasticity, activity-dependent neural development, learning, and memory in the mammalian brain Annu. Rev. Genet. 31 527-546
  • [5] Ticku M. K.(1993)Gene targeting and the biology of learning and memory Trends Neurosci. 16 521-527
  • [6] Chen C.(1997)NMDA-receptor-dependent synaptic plasticity: Multiple forms and mechanisms Neuron 18 839-842
  • [7] Tonegawa S.(1998)A kinase to remember: Dual roles for MAP kinase in long-term memory Neuron 20 895-904
  • [8] Silva A. J.(1991)A synaptic Ras-GTPase activating protein (p135 Syn GAP) inhibited by CaM kinase II Cell Reg. 2 357-371
  • [9] Smith A. M.(1995)Identification of multiple extracellular signal-regulated kinases (ERKs) with antipeptide antibodies J. Biol. Chem. 270 27489-27494
  • [10] Giese K. P.(1995)PD 098059 is a specific inhibitor of the activation of mitogen-activated protein kinase in vitro and in vivo EMBO J. 14 674-684
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