Primer development to obtain complete coding sequence of HA and NA genes of influenza A/H3N2 virus

被引：4

作者：

Agustiningsih A. ^{[1
]}

Trimarsanto H. ^{[2
,3
]}

Setiawaty V. ^{[1
]}

Artika I.M. ^{[2
,4
]}

Muljono D.H. ^{[2
,5
]}

机构：

[1] National Institute of Health Research and Development, Ministry of Health Republic of Indonesia, Jl. Percetakan Negara No.23, Jakarta

[2] Eijkman Institute for Molecular Biology, Jl. Diponegoro No.69, Jakarta

[3] Agency for the Assessment and Application of Technology, Jl. M. H. Thamrin 8, Jakarta

[4] Department of Biochemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Darmaga Campus, Bogor, West Java

[5] Faculty of Medicine, Hasanuddin University, Jl. Perintis Kemerdekaan Km 10, Makassar, South Sulawesi

来源：

BMC Research Notes | / 9卷 / 1期

关键词：

HA gene; Influenza A/H3N2 virus; NA gene; Primer set;

D O I：

10.1186/s13104-016-2235-8

中图分类号：

学科分类号：

摘要：

Background: Influenza is an acute respiratory illness and has become a serious public health problem worldwide. The need to study the HA and NA genes in influenza A virus is essential since these genes frequently undergo mutations. This study describes the development of primer sets for RT-PCR to obtain complete coding sequence of Hemagglutinin (HA) and Neuraminidase (NA) genes of influenza A/H3N2 virus from Indonesia. The primers were developed based on influenza A/H3N2 sequence worldwide from Global Initiative on Sharing All Influenza Data (GISAID) and further tested using Indonesian influenza A/H3N2 archived samples of influenza-like illness (ILI) surveillance from 2008 to 2009. Results: An optimum RT-PCR condition was acquired for all HA and NA fragments designed to cover complete coding sequence of HA and NA genes. A total of 71 samples were successfully sequenced for complete coding sequence both of HA and NA genes out of 145 samples of influenza A/H3N2 tested. Conclusions: The developed primer sets were suitable for obtaining complete coding sequences of HA and NA genes of Indonesian samples from 2008 to 2009. © 2016 The Author(s).

引用

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