Isolation and identification of cutaneous leishmaniasis species by PCR–RFLP in Ilam province, the west of Iran

被引：15

作者：

Kermanjani A. ^{[1
]}

Akhlaghi L. ^{[1
]}

Oormazdi H. ^{[1
]}

Hadighi R. ^{[1
]}

机构：

[1] Department of Parasitology, Faculty of Medicine, Iran University of Medical Sciences, Tehran

来源：

Journal of Parasitic Diseases | 2017年 / 41卷 / 1期

关键词：

Ilam province; Iran; L; major; PCR RFLP;

D O I：

10.1007/s12639-016-0772-7

中图分类号：

学科分类号：

摘要：

Cutaneous leishmaniasis (CL) is one of the most common parasitic diseases and public health problems in Iran. CL is endemic in most parts of Ilam province, in the west of Iran. The distance from the center of country, the great number of divers rural areas, and lack of specialists and laboratory facilities have been the major causes of Leishmania species remaining unknown in this region. Polymerase chain reaction followed by restriction fragment length polymorphism was performed to identify the Leishmania species in 61 patients with cutaneous lesions. Eventually L. major was confirmed as the cause of cutaneous leishmaniasis in Ilam province, the west of Iran. © 2016, Indian Society for Parasitology.

引用

页码：175 / 179

页数：4

共 23 条

[1]

Al-Jawabreh A., Schonian G., Hamarsheh O., Presber W., Clinical diagnosis of cutaneous leishmaniasis: a comparison study between standardized graded direct microscopy and ITS1-PCR of Giemsa-stained smears, Acta Trop, 99, 1, pp. 55-61, (2006)

[2]

Andresen K., Gaafar A., El-Hasan A., Ismail A., Dafaila M., Theander T.G., Kharazmi A., Evaluation of the polymerase chain reaction in the diagnosis of cutaneous leishmaniasis due to Leishmania major: a comparison with direct microscopy of smears and sections from lesions, Trans R Soc Trop Med Hyg, 90, pp. 133-135, (1996)

[3]

Ardehali S., Moattari A., Hatam G.R., Hosseini S.M.H., Sharifi I., Characterization of Leishmania isolated in Iran: 1. Serotyping with species specific monoclonal antibodies, Acta Trop, 75, 3, pp. 301-307, (2000)

[4]

Aviles H., Belli A., Armijos R., Monroy F., Harris E., PCR detection and identification of Leishmania parasites in clinical specimens in Ecuador: a comparison with classical diagnostic methods, J Parasitol, 85, pp. 181-187, (1999)

[5]

Bailey M.S., Lockwood D.N.J., Cutaneous leishmaniasis, Clin Dermatol, 25, pp. 203-211, (2007)

[6]

Daliva A.M., Momen H., Internal-trascribed-spacer (ITS) sequences used to explore polygenetic relationships within Leishmania, Ann Trop Med Parasitol, 94, 6, pp. 651-654, (2002)

[7]

Doudi M., Hejazi H., Razavi M., Narimani M., Khanjani S., Eslami G., Comparative molecular epidemiology of Leishmania major and Leishmania tropica by PCR-RFLP technique in hyper endemic cities of Isfahan and Bam, Iran, Med Sci Monit, 16, 11, pp. CR530-CR535, (2010)

[8]

Evans D., Handbook on isolation, characterization and cryopreservation of Leishmania, (1989)

[9]

Hajjaran H., Mohebali M., Razavi M.R., Et al., Identification of Leishmania species isolated from human cutaneous leishmaniasis, using random amplified polymorphic DNA (RAPD-PCR), Iran J Publ Health, 33, pp. 8-15, (2004)

[10]

Kassiri H., Sharifinia N., Jalilian M., Shemshad K., Epidemiological aspects of cutaneous leishmaniasis in Ilam province, west of Iran (2000–2007), Asian Pac J Trop Dis, 2, pp. 382-386, (2012)

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