Monoclonal antibody ONS-M21 recognizes integrin α3 in gliomas and medulloblastomas

被引：0
作者：
H Kishima
K Shimizu
K Tamura
Y Miyao
E Mabuchi
E Tominaga
J Matsuzaki
T Hayakawa
机构：
[1] Osaka University Medical School,Department of Neurosurgery
[2] 2-2 Yamadaoka,undefined
[3] Suita,undefined
[4] Exploratory Research Laboratory Chugai Pharmaceutical Co. Ltd,undefined
[5] 1-135 Komakado,undefined
来源：
British Journal of Cancer | 1999年 / 79卷
关键词：
glioma; medulloblastoma; monoclonal antibody; integrin α3;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The monoclonal antibody ONS-M21 recognizes an antigen found on the surface of glioma and medulloblastoma cells but does not react with the antigens of normal brain tissue. We purified and identified the 140-kDa protein by means of an antibody-binding affinity column. This 140-kDa antigen has sequences homologous to the amino-terminal region and five parts of the internal domain of integrin α3. When the integrin α3-related sequences was amplified and used to analyse the mRNA of glioma and medulloblastoma surgical specimens, the transcription level of integrin α3 mRNA appeared to be quantitatively correlated with the grade of malignancy. These findings suggest that the ONS-M21 antibody, which reacts with integrin α3, might be useful in the diagnosis of gliomas and medulloblastomas.
引用
收藏
页码：333 / 339
页数：6
相关论文
共 191 条
[1]  
Boku N(1995)Expression of integrin α3 in gastric and colorectal cancers: Its relation to wall construction and mode of invasion Jpn J Cancer Res 86 934-940
[2]  
Yoshida S(1993)Development of a method to measure kinetics of radiolabelled monoclonal antibody in human tumor with applications to microdosimetry: Positron emission tomography studies of iodine-124 labeled 3F8 monoclonal antibody in glioma Eur J Nucl Med 20 402-409
[3]  
Ohtsu A(1992)Human antiglioma monoclonal antibodies from patients with astrocytic tumors J Neurosurg 76 660-669
[4]  
Fujii T(1995)Human monoclonal antibody BT32/A6 and a cell cycle-independent glioma-associated surface antigen J Neurosurg 82 475-480
[5]  
Koba I(1995)Monoclonal antibody against human glioblastoma multiforme (U-87MG) immunoprecipitates a protein of molecular weight 38 kDa and inhibits tumor growth in nude mice J Neuroimmunol 56 91-98
[6]  
Oda Y(1988)Grading of astrocytoma. A simple and reproducible method Cancer 62 2152-2165
[7]  
Ryu M(1991)Receptor function for the integrin VLA-3: Fibronectin, collagen, and laminin binding are differentially influenced by ARG-GLY-ASP peptide and by divalent cations J Cell Biol 112 168-181
[8]  
Matsumoto T(1990)The treatment of medulloblastoma. Results of a prospective randomized trial of radiation therapy with and without CCNU, vincristine, and predonisone J Neurosurg 72 572-582
[9]  
Hasebe T(1996)A phase I study of an anti-epidermal growth factor receptor monoclonal antibody for the treatment of malignant gliomas Neurosurgery 39 478-483
[10]  
Hosokawa K(1984)Cell surface antigen of human bladder cancer defined by mouse monoclonal antibody Proc Natl Acad Sci USA 81 224-228
12345678910