Single nucleotide polymorphisms concordant with the horned/polled trait in Holsteins

被引：16

作者：

Cargill E.J. ^{[1
]}

Nissing N.J. ^{[1
]}

Grosz M.D. ^{[1
]}

机构：

[1] Monsanto Company, St Louis, MO 63167

来源：

BMC Research Notes | / 1卷 / 1期

关键词：

microRNA Target; Polymorphism Detection; Holstein Bull; Putative Regulatory Element; microRNA Family;

D O I：

10.1186/1756-0500-1-128

中图分类号：

学科分类号：

摘要：

Background. Cattle that naturally do not grow horns are referred to as polled, a trait inherited in a dominant Mendelian fashion. Previous studies have localized the polled mutation (which is unknown) to the proximal end of bovine chromosome 1 in a region approximately 3 Mb in size. While a polled genetic test, Tru-Polled, is commercially available from MetaMorphix Inc., Holsteins are not a validated breed for this test. Findings. Approximately 160 kb were sequenced within the known polled region from 12 polled and 12 horned Holsteins. Analysis of the polymorphisms identified 13 novel single nucleotide polymorphisms (SNPs) that are concordant with the horned/polled trait. Three of the 13 SNPs are located in gene coding or regulatory regions (e.g., the untranslated region, or UTR) where one is located in the 3'UTR of a gene and the other two are located in the 5'UTR and coding region (synonymous SNP) of another gene. The 3'UTR of genes have been shown to be targets of microRNAs regulating gene expression. In silico analysis indicates the 3'UTR SNP may disrupt a microRNA target site. Conclusion. These 13 novel SNPs concordant with the horned/polled trait in Holsteins represent a test panel for the breed and this is the first report to the authors' knowledge of SNPs within gene coding or regulatory regions concordant with the horned/polled trait in cattle. These SNPs will require further testing for verification and further study to determine if the 3'UTR SNP may have a functional effect on the polled trait in Holsteins. © 2008 Cargill et al; licensee BioMed Central Ltd.

引用

共 30 条

[1]

White W.T., Ibsen H.L., Horn inheritance in Galloway-Holstein cattle crosses, J Gen, 32, pp. 33-49, (1936)

[2]

Long C.R., Gregory K.E., Inheritance of the horned scurred, and polled condition in cattle, J Hered, 69, pp. 395-400, (1978)

[3]

Roeber D.L., Mies P.D., Smith C.D., Belk K.E., Field T.G., Tatum J.D., Scanga J.A., Smith G.C., National market-cow and bull beef quality audit 1999: A survey of producer-related defects in market cow and bulls, J Ani Sci, 79, pp. 658-665, (2001)

[4]

McKenna D.R., Roeber D.L., Bates P.K., Schmidt T.B., Hale D.S., Griffin D.B., Savell J.W., Brooks J.C., Morgan J.B., Montgomery T.H., Belk K.E., Smith G.C., National beef quality audit 2000: Survey of targeted cattle and carcass characteristics related to quality, quantity, and value of fed steers and heifers, J Ani Sci, 80, pp. 1212-1222, (2002)

[5]

Graf B., Senn M., Behavioural and physiological responses of calves to dehorning by heat cauterization with or without local anaesthesia, App Ani Behav Sci, 62, pp. 153-171, (1999)

[6]

MetaMorphix Inc

[7]

Georges M., Drinkwater R., King T., Mishra A., Moore S.S., Nielsen D., Sargeant L.S., Sorensen A., Steele M.R., Zhao X., Womack J.E., Hetzel J., Microsatellite mapping of a gene affecting horn development in Bos taurus, Nat Gen, 4, pp. 206-210, (1993)

[8]

Schmutz S.M., Marquess F.L.S., Berryere T.G., Moker J.S., DNA marker assisted selection of the polled condition in Charolais cattle, Mamm Gen, 6, pp. 710-713, (1995)

[9]

Brenneman R.A., Davis S.K., Sander J.O., Burns B.M., Wheeler T.C., Turner J.W., Taylor J.F., The polled locus maps to BTA1 in a Bos indicus × Bos taurus cross, J Hered, 87, pp. 156-161, (1996)

[10]

Harlizius B., Tammen I., Eichler K., Eggen A., Hetzel D.J., New markers on bovine chromosome 1 are closely linked to the polled gene in Simmental and Pinzgauer cattle, Mamm Gen, 8, pp. 255-257, (1997)

← 1 2 3 →