Lung responses to secondary endotoxin challenge in rats exposed to pig barn air

被引：8
作者：
Charavaryamath C. [1 ,2 ]
Keet T. [2 ]
Aulakh G.K. [2 ]
Townsend H.G.G. [3 ]
Singh B. [1 ,2 ]
机构：
[1] Immunology and Infectious Disease Research Group, University of Saskatchewan, Saskatoon
[2] Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon
[3] Department of Large Animal Clinical Sciences, University of Saskatchewan, Saskatoon
来源：
Journal of Occupational Medicine and Toxicology | / 3卷 / 1期
基金：
加拿大自然科学与工程研究理事会; 加拿大健康研究院;
关键词：
Lung Inflammation; Airway Epithelium; Hank Balance Salt Solution; Acute Lung Inflammation; Perivascular Edema;
D O I：
10.1186/1745-6673-3-24
中图分类号：
学科分类号：
摘要：
Background. Swine barn air contains endotoxin and many other noxious agents. Single or multiple exposures to pig barn air induces lung inflammation and loss of lung function. However, we do not know the effect of exposure to pig barn air on inflammatory response in the lungs following a secondary infection. Therefore, we tested a hypothesis that single or multiple exposures to barn air will result in exaggerated lung inflammation in response to a secondary insult with Escherichia coli LPS (E. coli LPS). Methods. We exposed Sprague-Dawley rats to ambient (N = 12) or swine barn air (N = 24) for one or five days and then half (N = 6/group) of these rats received intravenous E. coli LPS challenge, observed for six hours and then euthanized to collect lung tissues for histology, immunohistochemistry and ELISA to assess lung inflammation. Results. Compared to controls, histological signs of lung inflammation were evident in barn exposed rat lungs. Rats exposed to barn air for one or five days and challenged with E. coli LPS showed increased recruitment of granulocytes compared to those exposed only to the barn. Control, one and five day barn exposed rats that were challenged with E. coli LPS showed higher levels of IL-1 in the lungs compared to respective groups not challenged with E. coli LPS. The levels of TNF- in the lungs did not differ among any of the groups. Control rats without E. coli LPS challenge showed higher levels of TGF-2 compared to controls challenged with E. coli LPS. Conclusion. These results show that lungs of rats exposed to pig barn air retain the ability to respond to E. coli LPS challenge. © 2008 Charavaryamath et al; licensee BioMed Central Ltd.
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