Development and initial characterization of 99mTc labeled N-acetyl neuraminic acid for its application in in-vivo imaging of cancer: a preclinical study

被引：0
作者：
Ravi Ranjan Kumar
Devinder Kumar Dhawan
Vijayta Dani Chadha
机构：
[1] Panjab University,Centre for Nuclear Medicine
[2] Panjab University,Department of Biophysics
来源：
Journal of Radioanalytical and Nuclear Chemistry | 2019年 / 322卷
关键词：
Sialic acid; Radiolabeling; Radionuclide imaging; Biodistribution; Technitium-99m; Experimentally induced colon tumor;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
In the present study, N-acetyl neuraminic acid (Neu5Ac) was labeled with 99mTc and its bioevaluation was performed. Radiolabeling of Neu5Ac was obtained with a maximum labeling yield of 93.6% and was confirmed by instant thin layer chromatography, mass spectra (ESI-MS) and high performance liquid chromatography. The resulting radiocomplex showed hydrophilic properties and stability up to 8 h at room temperature in rat serum and saline. Biodistribution and scintigraphic studies exhibited accumulation of activity in the colon of tumor bearing rats. In conclusion, [99mTc]Tc-N-acetyl neuraminic acid exhibits selectivity for neoplastic colon tissue.
引用
收藏
页码：533 / 543
页数：10
相关论文
共 101 条
[1]  
Feijoo C(1997)Sialic acid level in serum and tissue from colorectal cancer patients Cancer Lett 112 155-160
[2]  
Páez de la Cadena M(1998)Sialic acid concentration in serum and tissue of endometrial cancer patients Eur J Obstet Gynecol Reprod Biol 76 211-215
[3]  
Rodriguez-Berrocal FJ(1983)Expression of alpha 2,6 sialylated sugar chain in normal and neoplastic colon tissues. Detection by digoxigenin conjugated Eur J Histochem 37 257-265
[4]  
Martinez-Zorzano VS(1988) aglutinin Clin Chim Acta 176 251-257
[5]  
Paszkowska A(1990)Sialic acid as a marker for differentiation between benign and malignant intracranial tumours Bioassays 12 223-230
[6]  
Berbec H(1997)Molecular changes in carbohydrate antigens associated with cancer Int Rev Cytol 175 137-240
[7]  
Semczuk A(2010)Sialic acid in molecular and cellular interaction Carbohydr Res 345 1377-1383
[8]  
Cybulski M(2005)Consequences of the expression of sialylated antigens in breast cancer Curr Drug Targets Cardiovasc Hematol Disord 5 39-64
[9]  
Dall’Olio F(1997)Cancer metastasis: characterization and identification of the behavior of metastatic tumor cells and the cell adhesion molecules, including carbohydrates World J Surg 2 773-776
[10]  
Trerè D(2006)Close correlation between increased sialyl-lewis (x) expression and metastasis in human gastric carcinoma J Cancer Mol 2 107-116
12345678910