Genome-wide identification and expression analysis of ammonium transporter 1 (AMT1) gene family in cassava (Manihot esculenta Crantz) and functional analysis of MeAMT1;1 in transgenic Arabidopsis

Nitrogen (N), a fundamental macronutrient for plant growth and development, is absorbed from the soil primarily in the form of ammonium (NH4+) and uptaken through a plant’s ammonium transporters (AMTs). While AMT proteins have been documented within diverse plant taxa, there has been no systematic analysis of their activity in cassava (Manihot esculenta Crantz), which is highly resistant to nitrogen deficiency. Here, we perform a comprehensive genome-wide analysis to identify and characterize the functional dynamics of cassava ammonium transporters 1 (MeAMT1). We identified a total of six AMT1 genes in the cassava genome (MeAMT1;1 to MeAMT1;6), the phylogenetic analysis of which fell into three distinct subgroups based on the conserved motifs and gene structures. Collinearity analysis showed that segmental duplication events played a key role in expansion of the MeAMT1 gene family. Synteny analysis indicated that two MeAMT1 genes were orthologous to Arabidopsis and rice. MeAMT1 promoters were additionally found to include various cis-acting elements related to light responsiveness, hormones, stress, and development processes. According to the RNA-seq data, the majority of MeAMT1 genes displayed specific patterns in the tested tissues. qRT-PCR revealed that all the tested MeAMT1 genes were up-regulated by low ammonium exposure. Furthermore, Arabidopis transformed with MeAMT1;1 gene grew well than wild-type plants in response to ammonium deficiency, suggesting that MeAMT1s play important role in response to low ammonium. Overall, our work lays the groundwork for new understanding of the AMT1 gene family in cassava and provides a basis for breeding efficient nitrogen use in other plants.