The N-terminal 62 amino acid residues of the coat protein of Tomato yellow leaf curl Thailand virus are responsible for DNA binding

被引：6
作者：
Pitaksutheepong C. [1 ]
Vimoltat A. [2 ]
Nathwong B. [1 ]
Attathom S. [3 ]
机构：
[1] National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Klong Luang, Pathumthani 12120, Phahonyothin Road
[2] Center for Agricultural Biotechnology, Kasetsart University, Kamphaengsaen Campus, Nakhon Pathom
[3] Department of Plant Pathology, College of Agriculture Kamphaengsaen, Kasetsart University, Nakhon Pathom
来源：
Journal of General Plant Pathology | 2007年 / 73卷 / 1期
关键词：
Coat protein; DNA-binding domain; Geminivirus; Tomato yellow leaf curl Thailand virus;
D O I：
10.1007/s10327-006-0317-5
中图分类号：
学科分类号：
摘要：
The DNA-binding activity and DNA-binding domain of Tomato yellow leaf curl Thailand virus coat protein were investigated. A full-length coat protein (CP) and two truncated derivatives lacking the amino (CPΔ1-62) and carboxyl (CPΔ126-257) termini were produced in Escherichia coli as fusion proteins to glutathione-S-transferase (GST). Southwestern analysis showed that GST-CP bound both single-stranded (ss) and double-stranded (ds) DNA, while GST-CPΔ126-257 interacted only with ssDNA. Neither ss nor dsDNA bound to GST-CPΔ1-62. The results suggested that a putative DNA-binding domain is located at the N-terminal 1-62 amino residues. © 2007 The Phytopathological Society of Japan and Springer-Verlag.
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页码：72 / 75
页数：3
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共 16 条
  • [1] Attathom S., Chiemsombat P., Kositratana W., Sae-Ung N., Complete nucleotide sequence and genome analysis of bipartite Tomato yellow leaf curl virus in Thailand, Kasetsart J (Nat Sci), 28, pp. 632-639, (1994)
  • [2] Hanley-Bowdoin L., Settlage S.B., Orozco B.M., Nagar S., Robertson D., Geminiviruses: Models for plant DNA replication, transcription and cell cycle regulation, Crit Rev Plant Sci, 18, pp. 76-106, (1999)
  • [3] Khey-Pour A., Bendahmane M., Matzeit M., Accotto G.P., Crespi S., Gronenborn B., Tomato yellow leaf curl virus from Sardinia is a whitefly-transmitted monopartite geminivirus, Nucl Acids Res, 19, pp. 6763-6769, (1991)
  • [4] Kirthi N., Savithri H.S., A conserved zinc finger motif in the coat protein of Tomato leaf curl Bangalore virus is responsible for binding to ssDNA, Arch Virol, 148, pp. 2369-2380, (2003)
  • [5] Kotlitzky G., Boulton M.I., Pitaksutheepong C., Davies J.W., Epel B., Intracellular and intercellular movement of Maize streak geminivirus V1 and V2 proteins transiently expressed as green fluorescent protein fusions, Virology, 274, pp. 32-38, (2000)
  • [6] Kunik T., Palanichelvam K., Czosnek H., Citovsky V., Gafni Y., Nuclear import of the capsid protein of Tomato yellow leaf curl virus (TYLCV) in plant and insect cells, Plant J, 13, pp. 393-399, (1998)
  • [7] Liu H., Boulton M.I., Davies J.W., Maize streak virus coat protein binds single and double-stranded DNA in vitro, J Gen Virol, 78, pp. 1265-1270, (1997)
  • [8] Liu H., Boulton M.I., Thomas C.L., Prior D.A., Oparka K.J., Davies J.W., Maize streak virus coat protein is karyophyllic and facilitates nuclear transport of viral DNA, Mol Plant Microbe Interact, 12, pp. 894-900, (1999)
  • [9] Navot N., Pichersky E., Zeidan M., Zamir D., Czosnek H., Tomato yellow leaf curl virus: A whitefly-transmitted geminivirus with a single genomic component, Virology, 185, pp. 151-161, (1991)
  • [10] Palanichelvam K., Kunik T., Citovsky V., Gafni Y., The capsid protein of Tomato yellow leaf curl virus binds cooperatively to single-stranded DNA, J Gen Virol, 79, pp. 2829-2833, (1998)
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