Microarray and biochemical analysis of bufalin-induced apoptosis of HL-60 Cells

被引：0
作者：
Ailiang Chen
Jian Yu
Liang Zhang
Yimin Sun
Yan Zhang
Hongyan Guo
Yuxiang Zhou
Keith Mitchelson
Jing Cheng
机构：
[1] Tsinghua University School of Medicine,Medical Systems Biology Research Center
[2] Tsinghua University,Department of Biological Sciences and Biotechnology
[3] National Engineering Research Center for Beijing Biochip Technology & Capital Bio Corporation,undefined
来源：
Biotechnology Letters | 2009年 / 31卷
关键词：
AP-1; Bufalin; HL-60; Microarray; NFκB;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Bufalin is a natural toxin with anti-leukemic properties. It induces cell differentiation and apoptosis, as well as increasing the sensitivity of leukemia cells to other chemotherapeutic agents. We investigated the biological effects and molecular mechanisms of bufalin triggered apoptosis in HL-60 cells by gene expression profiling. The broad transcriptional response to bufalin was consistent with bufalin’s action of regulating HL-60 cell proliferation and apoptosis, as well as its synergistic effect with other drugs. Further transcription factor ELISA experiments suggested that the transcription factors NFκB and AP-1 were activated to promote bufalin-induced HL-60 cell apoptosis. Our study provides new insights into the molecular mechanisms of bufalin, might prove to be beneficial in leukemia therapy.
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页码：487 / 494
页数：7
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