Solid phase subtractive cloning in differentially expressed genes identification

被引：0
作者：
Oscar F. D’Urso
Pietro I. D’Urso
Carlo Storelli
Santo Marsigliante
机构：
[1] Promeridio Association,Section of Neurosurgery, Department of Neurosciences
[2] Non-Profit Research Association,Department of Biological and Environmental Sciences and Technologies (DiSTeBA)
[3] University of Bari Medical School,undefined
[4] University of Lecce,undefined
来源：
Molecular Biology Reports | 2010年 / 37卷
关键词：
Subtractive cloning; Microarray; Solid surface; Library; Sequencing; Differentially expressed;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
We developed an array-based subtractive hybridization system for one-step high-throughput subtraction. We printed subtractor RNA up to 10.000 times obtaining an excellent contact surface using a little amount of RNA. During hybridization cDNA, common to subtractor and target samples, remains attached to slide immobilized RNA, leaving free in solution target specific cDNA which after retrieval is cloned.
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页码：1435 / 1438
页数：3
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