Solid phase subtractive cloning in differentially expressed genes identification

被引:0
作者
Oscar F. D’Urso
Pietro I. D’Urso
Carlo Storelli
Santo Marsigliante
机构
[1] Promeridio Association,Section of Neurosurgery, Department of Neurosciences
[2] Non-Profit Research Association,Department of Biological and Environmental Sciences and Technologies (DiSTeBA)
[3] University of Bari Medical School,undefined
[4] University of Lecce,undefined
来源
Molecular Biology Reports | 2010年 / 37卷
关键词
Subtractive cloning; Microarray; Solid surface; Library; Sequencing; Differentially expressed;
D O I
暂无
中图分类号
学科分类号
摘要
We developed an array-based subtractive hybridization system for one-step high-throughput subtraction. We printed subtractor RNA up to 10.000 times obtaining an excellent contact surface using a little amount of RNA. During hybridization cDNA, common to subtractor and target samples, remains attached to slide immobilized RNA, leaving free in solution target specific cDNA which after retrieval is cloned.
引用
收藏
页码:1435 / 1438
页数:3
相关论文
共 69 条
[1]  
Sagerstrom CG(1997)Subtractive cloning: past, present, and future Annu Rev Biochem 66 751-783
[2]  
Sun BI(1984)Cell-type-specific cDNA probes and the murine I region: the localization and orientation of A Proc Natl Acad Sci USA 81 2194-2198
[3]  
Sive HL(1993), a potential mediator of p53 tumor suppression Cell 75 817-825
[4]  
Davis MM(1995)A novel and simple methodology to generate subtracted cDNA libraries Nucleic Acids Res 23 2565-2566
[5]  
Cohen DI(1983)Activation of mouse genes in transformed cells Cell 34 557-567
[6]  
Nielsen EA(1981)The isolation of ecdysterone inducible genes by hybridization subtraction chromatography Nucleic Acids Res 9 1191-1202
[7]  
Steinmetz M(1991)Subtractive cDNA cloning using oligo(dt) Nucleic Acids Res 19 7097-7104
[8]  
Paul WE(1992)-latex and PCR: isolation of cDNA clones specific to undifferentiated human embryonal carcinoma cells Nucleic Acids Res 20 3528-333
[9]  
Hood L(1993)A novel method for the isolation of tissue-specific genes Anal Biochem 208 330-361
[10]  
El-Deiry WS(1976)A subtractive hybridization method to isolate tissue-specific transcripts: application to the selection of new brain-specific products Annu Rev Biophys Bioeng 5 337-951