Comparative analysis of growth characteristics of Sprague Dawley rats obtained from different sources

被引：51

作者：

Marcia Brower

Martha Grace

Catherine M. Kotz

Vijay Koya

机构：

[1] Boston Scientific Corporation, 4100 Hamline Avenue North, St. Paul, 55112-5798, MN

[2] Veteran Affairs Medical Center, Minnesota Obesity Center, Minneapolis, MN

来源：

Laboratory Animal Research | 2015年 / 31卷 / 4期

关键词：

genetics; outbreds; phenotype; Sprague Dawley;

D O I：

10.5625/lar.2015.31.4.166

中图分类号：

学科分类号：

摘要：

Genetic background in animal models is an intrinsic research variable in biomedical research. Although inbred strains offer genetic uniformity, the outbred stocks, known for genetic variability are often used to develop animal models of human disease. The genetic variability is considered to be even higher when outbred stocks are obtained from different sources. In order to examine the degree of variability of an outbred stock obtained from various sources, Sprague Dawley (SD) rat lines obtained from two sources were evaluated for their growth characteristics. The SD rats from Charles River laboratories (CRL) and Harlan Laboratories (HAR) were monitored for weight gain from the age of 6 weeks to 24 weeks. Food intake was monitored between 13 and 24 weeks. Body composition, organ weights, tibial lengths and blood parameters were measured. There was no difference observed in food intake per 100 gram body weight at most of the time points. CRL rats showed higher body fat mass (49.6%), higher gross liver weights (22.2%), lower testicular weights (30.8%) and lower cholesterol levels (25.4%) than HAR rats. Phenotypic differences may be attributed to genetic heterogeneity of the SD outbred stock between the two sources and represent a significant research variable impacting studies especially related to metabolic diseases. Therefore, in order the minimize research variables for those studies where genetic diversity is not a basis for experimental design, the use of single source genetically uniform inbred animal models is highly recommended over the use of outbred stocks. © 2015, BioMed Central Ltd.

引用

页码：166 / 173

页数：7

共 23 条

[1]

Benson V.L., McMahon A.C., Lowe H.C., Khachigian L.M., The streptozotocin-treated Sprague-Dawley rat: a useful model for the assessment of acute and chronic effects of myocardial ischaemia reperfusion injury in experimental diabetes, Diab Vasc Dis Res, 4, 2, pp. 2-153, (2007)

[2]

Chia R., Achilli F., Festing M.F., Fisher E.M., The origins and uses of mouse outbred stocks, Nat Genet, 37, 11, pp. 11-1181, (2005)

[3]

Ciccotosto G.D., Hand T.A., Mains R.E., Eipper B.A., Breeding stock-specific variation in peptidylglycine alpha-amidating monooxygenase messenger ribonucleic acid splicing in rat pituitary, Endocrinology, 141, 2, pp. 2-476, (2000)

[4]

Crous-Bou M., Rennert G., Salazar R., Rodriguez-Moranta F., Rennert H.S., Lejbkowicz F., Kopelovich L., Lipkin S.M., Gruber S.B., Moreno V., Genetic polymorphisms in fatty acid metabolism genes and colorectal cancer, Mutagenesis, 27, 2, pp. 2-169, (2012)

[5]

Davidson E.P., Coppey L.J., Calcutt N.A., Oltman C.L., Yorek M.A., Diet-induced obesity in Sprague-Dawley rats causes microvascular and neural dysfunction, Diabetes Metab Res Rev, 26, 4, pp. 4-306, (2010)

[6]

Festing M.F., Reduction in animal use in the production and testing of biologicals, Dev Biol Stand, 101, pp. 195-200, (1999)

[7]

Fox J.G., Anderson L.C., Loew F.M., Quimby F.W., Laboratory Animal Medicine.

[8]

Gear R.B., Yan M., Schneider J., Succop P., Heffelfinger S.C., Clegg D.J., Charles River Sprague Dawley rats lack early age-dependent susceptibility to DMBA-induced mammary carcinogenesis, Int J Biol Sci, 3, 7, pp. 7-408, (2007)

[9]

Heikkila R.E., Differential neurotoxicity of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in Swiss-Webster mice from different sources, Eur J Pharmacol, 117, 1, pp. 1-131, (1985)

[10]

Hubrecht R.C., Kirkwood J., The UFAW Handbook on the Care and Management of Laboratory and Other Research Animals, (2010)

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