Exploration of sulfur metabolism in the yeast Kluyveromyces lactis

被引:0
|
作者
Agnès Hébert
Marie-Pierre Forquin-Gomez
Aurélie Roux
Julie Aubert
Christophe Junot
Valentin Loux
Jean-François Heilier
Pascal Bonnarme
Jean-Marie Beckerich
Sophie Landaud
机构
[1] AgroParisTech,UMR MICALIS, INRA
[2] AgroParisTech,INRA
[3] UMR 782 Génie et Microbiologie des Procédés Alimentaires,CEA, Service de Pharmacologie et d’Immunoanalyse
[4] Centre de Biotechnologies Agro-Industrielles,UMR 518 Mathématiques et Informatiques Appliquées
[5] DSV/iBiTec-S,INRA
[6] CEA/Saclay,AgroParisTech
[7] AgroParisTech,undefined
[8] INRA,undefined
[9] INRA,undefined
[10] Unité Mathématique,undefined
[11] Informatique et Génome UR1077,undefined
[12] Université Catholique de Louvain,undefined
[13] Louvain Center for Toxicology and Applied Pharmacology (LTAP),undefined
[14] UMR 782 Génie et Microbiologie des Procédés Alimentaires,undefined
[15] Centre de Biotechnologies Agro-Industrielles,undefined
来源
Applied Microbiology and Biotechnology | 2011年 / 91卷
关键词
Sulfur metabolism; Volatile sulfur compounds; Transcriptome; Metabolome;
D O I
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学科分类号
摘要
Hemiascomycetes are separated by considerable evolutionary distances and, as a consequence, the mechanisms involved in sulfur metabolism in the extensively studied yeast, Saccharomyces cerevisiae, could be different from those of other species of the phylum. This is the first time that a global view of sulfur metabolism is reported in the biotechnological yeast Kluyveromyces lactis. We used combined approaches based on transcriptome analysis, metabolome profiling, and analysis of volatile sulfur compounds (VSCs). A comparison between high and low sulfur source supplies, i.e., sulfate, methionine, or cystine, was carried out in order to identify key steps in the biosynthetic and catabolic pathways of the sulfur metabolism. We found that sulfur metabolism of K. lactis is mainly modulated by methionine. Furthermore, since sulfur assimilation is highly regulated, genes coding for numerous transporters, key enzymes involved in sulfate assimilation and the interconversion of cysteine to methionine pathways are repressed under conditions of high sulfur supply. Consequently, as highlighted by metabolomic results, intracellular pools of homocysteine and cysteine are maintained at very low concentrations, while the cystathionine pool is highly expandable. Moreover, our results suggest a new catabolic pathway for methionine to VSCs in this yeast: methionine is transaminated by the ARO8 gene product into 4-methylthio-oxobutyric acid (KMBA), which could be exported outside of the cell by the transporter encoded by PDR12 and demethiolated by a spontaneous reaction into methanethiol and its derivatives.
引用
收藏
页码:1409 / 1423
页数:14
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