Phenotyping of Porcine Apolipoprotein E Using Isoelectric Focusing and Localization of the APOE Gene Within the Halothane-Susceptibility Linkage Group

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作者：
Jolanta Kuryl
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来源：
Biochemical Genetics | 1999年 / 37卷
关键词：
APOLIPOPROTEIN E; ISOELECTRIC FOCUSING; IMMUNOBLOTTING; GENE LINKAGE;
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摘要：
A method has been developed for the phenotypingof pig apolipoprotein E. It was based on isoelectricfocusing of sialidase-treated and delipidated bloodplasma followed by immunoblotting. As the first antibody the anti-human apolipoprotein E antibody wasused. The genetic polymorphism of pig apolipoprotein Eappeared to be controlled by three alleles at one locus.A two-point linkage analysis was performed between certain loci belonging to the halothane linkagegroup and the APOE locus on material comprising 9Zlotnicka Spotted boars, 30 Polish Large White sows, andtheir 160 offspring. A tight linkage between the A1BG and the APOE loci was documented (Θ= 0.038).
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页码：281 / 287
页数：6
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