In Vitro Analysis of the Co-Assembly of Type-I and Type-III Collagen

被引：0
作者：
Esma Eryilmaz
Winfried Teizer
Wonmuk Hwang
机构：
[1] Texas A&M University,Department of Physics and Astronomy
[2] Selcuk University,Department of Biotechnology, College of Science
[3] Texas A&M University,Departments of Physics and Astronomy and Materials Science & Engineering
[4] Tohoku University,WPI Advanced Institute for Materials Research
[5] Texas A&M University,Department of Biomedical Engineering and Materials Science & Engineering
[6] Korea Institute for Advanced Study,School of Computational Sciences
来源：
Cellular and Molecular Bioengineering | 2017年 / 10卷
关键词：
AFM; Biomimetic surface; Collagen; Extracellular matrix; Heterogeneous assembly; Macromolecular assembly;
D O I：
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中图分类号：
学科分类号：
摘要：
An important step towards achieving functional diversity of biomimetic surfaces is to better understand the co-assembly of the extracellular matrix components. For this, we study type-I and type-III collagen, the two major collagen types in the extracellular matrix. By using atomic force microscopy, custom image analysis, and kinetic modeling, we study their homotypic and heterotypic assembly. We find that the growth rate and thickness of heterotypic fibrils decrease as the fraction of type-III collagen increases, but the fibril nucleation rate is maximal at an intermediate fraction of type-III. This is because the more hydrophobic type-I collagen nucleates fast and grows in both longitudinal and lateral directions, whereas more hydrophilic type-III limits lateral growth of fibrils, driving more monomers to nucleate additional fibrils. This demonstrates that subtle differences in physico-chemical properties of similar molecules can be used to fine-tune their assembly behavior.
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页码：41 / 53
页数：12
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