Somatic embryogenesis and plant regeneration in zygotic embryo cultures of balloon flower

被引：0
|
作者：
Suk W. Kim
Jang R. Liu
机构：
[1] Gene Bank,Genetic Resources Center
[2] Korea Research Institute of Bioscience and Biotechnology (KRIBB),Plant Cell Biotechnology Laboratory
来源：
Plant Cell, Tissue and Organ Culture | 1999年 / 58卷
关键词：
Campanulaceae; somatic embryo; suspension culture;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Mature zygotic embryos of balloon flower (Platycodon grandiflorum) formed embryogenic calluses at a frequency of 43% when cultured on Murashige and Skoog medium supplemented with 4.52 μM 2,4-dichlorophenoxyacetic acid (2,4-D). Cell suspension cultures were established from embryogenic calluses using MS liquid medium with 4.52 μM 2,4-D. Following transfer to solid MS basal medium, cell suspension cultures gave rise to somatic embryos, which then developed into plantlets. Plantlets were transplanted to potting soil and grown to maturity.
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页码：227 / 230
页数：3
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