Quenched Emission of Fluorescence by Ligand Functionalized Gold Nanoparticles

被引：0
作者：
Kadir Aslan
Víctor H. Pérez-Luna
机构：
[1] Illinois Institute of Technology,Department of Chemical and Environmental Engineering
来源：
Journal of Fluorescence | 2004年 / 14卷
关键词：
Gold nanoparticles; self-assembled monolayers; surface modification; fluorescence; energy transfer;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A fluorescence-based detection scheme that uses ligand functionalized gold nanoparticles is proposed. The transduction scheme is based on the strong quenching of the fluorescence emission exerted by metallic surfaces on fluorophores positioned in their immediate vicinity (<5 nm). Binding of fluorophore-labeled anti-biotin to biotinylated gold nanoparticles resulted in decreased fluorescence emission intensity. Subsequent competitive dissociation of labeled anti-biotin with D-biotin resulted in increased fluorescence emission intensity. These interactions occurred by means of specific molecular recognition because when the binding sites of anti-biotin were saturated with D-biotin prior to interaction with the gold nanoparticles; changes in the fluorescence emission intensity were not observed.
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页码：401 / 405
页数：4
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共 55 条
[1]  
Storhoff J. J.(1998)One-pot colorimetric differentiation of polynucleotides with single base imperfections using gold nanoparticle probes J. Am. Chem. Soc. 120 1959-1964
[2]  
Elghanian R.(1996)A DNA-based method for rationally assembling nanoparticles into macroscopic materials Nature 382 607-609
[3]  
Mucic R. C.(2000)Conductimetric membrane strip immunosensor with polyaniline-bound gold colloids as signal generator Biosens.Bioelectron. 14 907-915
[4]  
Mirkin C. A.(1999)In situ assembly of colloidal particles into miniaturized biosensors Langmuir 15 3693-3698
[5]  
Letsinger R. L.(1999)Catalytic asymmetric dihydroxylation by gold colloids functionalized with self-assembled monolayers Langmuir 15 4957-4959
[6]  
Mirkin C. A.(1998)Nanocrystal Superlattices Annu. Rev. Phys. Chem. 49 371-404
[7]  
Letsinger R. L.(1984)Excited states at metal surfaces and their non-radiative relaxation J. Phys. Chem. 88 837-848
[8]  
Mucic R. C.(1984)Dynamical processes at surfaces: Excitation of electron-hole pairs Phys. Rev. B 29 4382-4394
[9]  
Storhoff J. J.(1993)Fluorescence properties of submonolayers of rhodamine 6G in front of a mirror J. Chem. Phys. 98 5276-5280
[10]  
Kim J. H.(2001)Radiative decay engineering: Biophysical and Biomedical applications Anal. Biochem. 298 1-24
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