Automated sample preparation for electrospray ionization mass spectrometry based on CLOCK-controlled autonomous centrifugal microfluidics

被引：0

作者：

Futami, Masahiro ^{[1
]}

Naito, Hiroki ^{[1
]}

Ninomiya, Satoshi ^{[2
]}

Chen, Lee Chuin ^{[2
]}

Iwano, Tomohiko ^{[3
]}

Yoshimura, Kentaro ^{[4
]}

Ukita, Yoshiaki ^{[2
]}

机构：

[1] Univ Yamanashi, Integrated Grad Sch Med Engn & Agr Sci, Dept Engn, 4-3-11 Takeda, Kofu 4008510, Japan

[2] Univ Yamanashi, Grad Fac Interdisciplinary Res, 4-3-11 Takeda, Kofu 4008510, Japan

[3] Univ Yamanashi, Fac Med, Dept Anat & Cell Biol, 1110 Shimokato, Chuo 4093898, Japan

[4] Univ Yamanashi, Ctr Med Educ & Sci, Interdisciplinary Grad Sch Med, Div Mol Biol, 1110 Shimokato, Chuo 4093898, Japan

来源：

BIOMEDICAL MICRODEVICES | 2024年 / 26卷 / 02期

关键词：

Centrifugal microfluidics; Mass spectrometry; Autonomous microfluidic control; Sample preparation; PLATFORM;

D O I：

10.1007/s10544-024-00703-4

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

We report a centrifugal microfluidic device that automatically performs sample preparation under steady-state rotation for clinical applications using mass spectrometry. The autonomous microfluidic device was designed for the control of liquid operation on centrifugal hydrokinetics (CLOCK) paradigm. The reported device was highly stable, with less than 7% variation with respect to the time of each unit operation (sample extraction, mixing, and supernatant extraction) in the preparation process. An agitation mechanism with bubbling was used to mix the sample and organic solvent in this device. We confirmed that the device effectively removed the protein aggregates from the sample, and the performance was comparable to those of conventional manual sample preparation procedures that use high-speed centrifugation. In addition, probe electrospray ionization mass spectrometry (PESI-MS) was performed to compare the device-treated and manually treated samples. The obtained PESI-MS spectra were analyzed by partial least squares discriminant analysis, and the preparation capability of the device was found to be equivalent to that of the conventional method.

引用

页数：9

共 32 条

[1] Partial least squares for discrimination [J].