Functional ectodomain of the hemagglutinin-neuraminidase protein is expressed in transgenic tobacco cells as a candidate vaccine against Newcastle disease virus

被引：0
作者：
Kok Song Lai
Khatijah Yusoff
Maziah Mahmood
机构：
[1] Universiti Putra Malaysia (UPM),Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences
[2] Universiti Putra Malaysia (UPM),Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences
[3] Universiti Putra Malaysia (UPM),Institute of Bioscience
来源：
Plant Cell, Tissue and Organ Culture (PCTOC) | 2013年 / 112卷
关键词：
Hemagglutinin-neuraminidase; Newcastle disease virus; ADH 5′-UTR; Plant-based vaccine;
D O I：
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学科分类号：
摘要：
Recently, the use of plants for the production of recombinant proteins has been well demonstrated with promising outcomes. In this study, an efficient Nicotiana tabacum L. cv. Bright Yellow 2 (BY-2) cells system expressing the ectodomain of hemagglutinin-neuraminidase (eHN) protein from Newcastle disease virus (NDV) strain AF2240 was established. Transgenic tobacco BY-2 cell cultures expressing the immunogenic eHN protein were generated and the translation efficiency of eHN protein was enhanced using the 5′-untranslated region of Nicotiana tabacumalcohol dehydrogenase gene (NtADH 5′-UTR) under the control of strong cauliflower mosaic virus (CaMV 35S) promoter. Transgenic lines verified by real-time PCR showed high level of eHN mRNA transcripts and immunoblotting confirmed the presence of 66 kD eHN protein. The eHN protein was stably produced in an average of 0.2–0.4 % total soluble protein. Green fluorescent protein-tagged eHN protein was expressed and localized at the cytosol of BY-2 cell. All mice receiving purified eHN protein from transgenic tobacco BY-2 cells produced specific anti-NDV antibodies. We concluded that plant made eHN elicit immune response and can serve as candidate vaccine against NDV.
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页码：117 / 121
页数：4
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