Identification of pedilanthus leaf curl virus and associated betasatellite infecting turnip in Pakistan

被引：0
作者：
Samrah Munir
Muhammad Khurshid
Hira Kanwal
Mujahid Hussain
Muhammad N. Sattar
Irfan Ali
Atiq-ur Rehman
Zafar Iqbal
机构：
[1] University of the Punjab,Institute of Biochemistry and Biotechnology
[2] King Faisal University,Department of Environment and Natural Resources, College of Agriculture and Food Science
[3] University of Agriculture,Centre of Agricultural Biochemistry and Biotechnology
[4] National Institute for Biotechnology and Genetic Engineering (NIBGE),Agricultural Biotechnology Division
[5] University of Florida,Department of Plant Pathology
来源：
Journal of Plant Pathology | 2018年 / 100卷
关键词：
Begomovirus; Betasatellite; Recombination;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Complete begomovirus and betasatellite genomes were isolated from turnip (Brassica rapa) plants exhibiting leaf crumpling symptoms in Pakistan. In this study, two complete begomovirus genomes and one betasatellite molecule were cloned, sequenced and characterized. The identified begomovirus isolates were 100% identical to each other and shared their highest nucleotide (nt) sequence identity at 94.4 and 92.7% with pedilanthus leaf curl virus (PeLCV) and tomato leaf curl Kerala virus (ToLCKeV), respectively. However, in phylogenetic analysis both isolates grouped separately in the ToLCKeV clade. The detailed phylogenetic and recombination analysis showed that the isolates have unique and recombinant nature thus, representing new isolates of PeLCV from turnip in Pakistan. The cloned betasatellite isolate (1371 nt in length) was a non-recombinant molecule and shared highest nt sequence identity (89.8%) with tobacco leaf curl betasatellite (TbLCB). This is the first report of a unique isolate of PeLCV associated with TbLCB infecting B. rapa in Pakistan. The present study extends our knowledge about the current diversity of PeLCV in the important crops.
引用
收藏
页码：317 / 321
页数：4
相关论文
共 153 条
  • [1] Akhter A(2009)A severe leaf curl disease on chilies in Pakistan is associated with multiple begomovirus components Plant Dis 93 962-1047
  • [2] Qazi J(2007)An exact nonparametric method for inferring mosaic structure in sequence triplets Genetics 176 1035-6765
  • [3] Saeed M(2004)Geminate structures of J Virol 78 6758-318
  • [4] Mansoor S(2002)Universal primers for the PCR-mediated amplification of DNA β: a molecule associated with some monopartite begomoviruses Mol Biotechnol 20 315-474
  • [5] Boni MF(2004)Diversity of DNA 1; a satellite-like molecule associated with monopartite begomovirus-DNA β complexes Virology 324 462-781
  • [6] Posada D(2008)Recommendations for the classification and nomenclature of the DNA-β satellites of begomoviruses Arch Virol 153 763-175
  • [7] Feldman MW(2010)Turnip curly top virus, a highly divergent geminivirus infecting turnip in Iran Virus Res 152 169-1598
  • [8] Böttcher B(2001)The core region of the coat protein gene is highly useful for establishing the provisional identification and classification of begomoviruses Arch Virol 146 1581-1619
  • [9] Unseld S(2015)Revision of Begomovirus taxonomy based on pairwise sequence comparisons Arch Virol 160 1593-86
  • [10] Ceulemans H(2003)Universal primers for the PCR-mediated amplification of DNA 1: a satellite-like molecule associated with begomovirus-DNA β complexes Mol Biotechnol 23 83-15
12345678910