Immobilized lipase enzyme on green synthesized magnetic nanoparticles using Psidium guava leaves for dye degradation and antimicrobial activities

被引:11
作者
Fahim, Yosri A. [1 ]
El-Khawaga, Ahmed M. [1 ]
Sallam, Reem M. [1 ,2 ]
Elsayed, Mohamed A. [3 ]
Assar, Mohamed Farag Ali [4 ]
机构
[1] Galala Univ, Fac Med, Dept Basic Med Sci, Galala City 43511, Suez, Egypt
[2] Ain Shams Univ, Fac Med, Dept Med Biochem & Mol Biol, Cairo 11566, Egypt
[3] Egyptian Armed Forces, Mil Tech Coll MTC, Chem Engn Dept, Cairo, Egypt
[4] Menoufia Univ, Fac Sci, Dept Chem, Biochem Div, Shibin Al Kawm, Menoufia, Egypt
关键词
Enzymes; Nanotechnology; Lipase; Magnetic nanoparticles; Immobilization; METHYLENE-BLUE; PHOTOCATALYTIC DEGRADATION; AQUEOUS-SOLUTION; REMOVAL; ANTIOXIDANT; INHIBITION; EFFICIENCY; BIOFILMS; CANCER; WATER;
D O I
10.1038/s41598-024-58840-y
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Zinc ferrite nanoparticles (ZnF NPs) were synthesized by a green method using Psidium guava Leaves extract and characterized via structural and optical properties. The surface of ZnF NPs was stabilized with citric acid (CA) by a direct addition method to obtain (ZnF-CA NPs), and then lipase (LP) enzyme was immobilized on ZnF-CA NPs to obtain a modified ZnF-CA-LP nanocomposite (NCs). The prepared sample's photocatalytic activity against Methylene blue dye (MB) was determined. The antioxidant activity of ZnF-CA-LP NCs was measured using 1,1-diphenyl-2-picryl hydrazyl (DPPH) as a source of free radicals. In addition, the antibacterial and antibiofilm capabilities of these substances were investigated by testing them against gram-positive Staphylococcus aureus (S. aureus ATCC 25923) and gram-negative Escherichia coli (E. coli ATCC 25922) bacterial strains. The synthesized ZnF NPs were discovered to be situated at the core of the material, as determined by XRD, HRTEM, and SEM investigations, while the CA and lipase enzymes were coated in this core. The ZnF-CA-LP NCs crystallite size was around 35.0 nm at the (311) plane. Results obtained suggested that 0.01 g of ZnF-CA-LP NCs achieved 96.0% removal of 5.0 ppm of MB at pH 9.0. In-vitro zone of inhibition (ZOI) and minimum inhibitory concentration (MIC) results verified that ZnF-CA-LP NCs exhibited its encouraged antimicrobial activity against S. aureus and E. coli (20.0 +/- 0.512, and 27.0 +/- 0.651 mm ZOI, respectively) & (1.25, and 0.625 mu g/ml MIC, respectively). ZnF-CA-LP NPs showed antibiofilm percentage against S. aureus (88.4%) and E. coli (96.6%). Hence, ZnF-CA-LP NCs are promising for potential applications in environmental and biomedical uses.
引用
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页数:20
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