Characterization and immunogenicity of bone marrow-derived mesenchymal stem cells under osteoporotic conditions

被引：0
作者：
Yingkang Huang
Yin Yin
Yanzheng Gu
Qiaoli Gu
Huilin Yang
Zhengyu Zhou
Qin Shi
机构：
[1] Orthopedic Institute of Soochow University,Orthopedic Department, the First Affiliated Hospital of Soochow University, Medical College of Soochow University
[2] Soochow University,Laboratory Animal Center, Medical College of Soochow University
[3] the First Affiliated Hospital of Soochow University,Clinical Immunology Institute of Jiangsu Province
[4] Chinese Ministry of Science and Technology,Key Laboratory of Stem Cells and Biomedical Materials of Jiangsu Province
来源：
Science China Life Sciences | 2020年 / 63卷
关键词：
mesenchymal stem cells; osteogenesis; chondrogenesis; adipogenesis; immunogenicity; osteoporosis;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Mesenchymal stem cells (MSCs) are characterized by their multilineage potential and low immunogenicity. However, the properties of MSCs under pathological conditions are unclear. The current study investigated the differentiation potential and immunological characteristics of bone marrow-derived MSCs from ovariectomized-osteoporotic rats (OP-BMSCs). Although the expression of cell morphology- and stemness-related surface markers was similar between OP-BMSCs and BMSCs from healthy rats (H-BMSCs), the proliferation rate was significantly decreased compared with that of H-BMSCs. Regarding multilineage potential, osteogenesis and chondrogenesis abilities of OP-BMSCs decreased, but the adipogenesis ability was significantly enhanced compared with that of H-BMSCs. As expected, decreased osteogenesis following osteogenic induction resulted in reduced expression of β-catenin, osteocalcin, and runt-related transcription factor 2 in OP-BMSCs. Remarkably, the expression of the co-stimulatory proteins CD40 and CD80 was significantly higher, whereas the expression of the negative co-stimulatory molecule programmed cell death ligand 1 was significantly lower in the OP-BMSCs than that in H-BMSCs. Consequently, H-BMSCs inhibited the proliferation and secretion of inflammatory cytokines from anti-CD3 antibody-activated T cells, whereas OP-BMSCs did not. These results indicate that decreased osteogenesis and increased immunogenicity of OP-BMSCs contribute to bone loss in osteoporosis.
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页码：429 / 442
页数：13
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