Complete Nucleotide Sequence of a Strawberry Isolate of Beet Pseudoyellows Virus

被引：0
作者：
Ioannis E. Tzanetakis
Robert R. Martin
机构：
[1] Oregon State University,Molecular and Cellular Biology Program, Department of Botany and Plant Pathology
[2] USDA-ARS,Horticultural Crops Research Laboratory
来源：
Virus Genes | 2004年 / 28卷
关键词：
dsRNA; pallidosis; strawberry;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
In our effort to identify the causal agent(s) of strawberry pallidosis we found a single pallidosis positive plant that did not give any amplicons after RT-PCR using primer sets representing multiple regions of Strawberry pallidosis associated virus (SPaV) genome and failed to react with antibodies directed against the recombinant coat protein (CP) of SPaV. DsRNA extracted from this plant showed a similar pattern to that of SPaV indicating that another crinivirus may infect strawberry. Sequence analysis of multiple cDNA clones corresponding to the heat shock 70 homolog gene (HSP70h) of the unknown virus indicated that it was Beet pseudoyellows virus (BPYV). Analysis of the complete nucleo- tide sequence of BPYV-strawberry revealed that this isolate has several distinct features when compared to Cucumber yellows virus (CuYV), a cucumber strain of BPYV, including an entire ORF not found in CuYV.
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页码：239 / 246
页数：7
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