Antifungal Activity of Magnesium Oxide Nanoparticles: Effect on the Growth and Key Virulence Factors of Candida albicans

被引：0
作者：
Fanzhi Kong
Jiaying Wang
Rui Han
Shuaiqi Ji
Jin Yue
Yongliang Wang
Lei Ma
机构：
[1] Affiliated Hospital of Qingdao University,Department of Stomatology
[2] School of Stomatology of Qingdao University,undefined
来源：
Mycopathologia | 2020年 / 185卷
关键词：
MgO NPs; Biofilm; Adhesion; Fungal virulence;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The aim of this research was to study the effects of different concentrations of magnesium oxide nanoparticles (MgO NPs) on the growth and key virulence factors of Candida albicans (C. albicans). The minimum inhibitory concentration (MIC) of MgO NPs against C. albicans was determined by the micro-broth dilution method. A time-kill curve of MgO NPs and C. albicans was established to investigate the ageing effect of MgO NPs on C. albicans. Crystal violet staining, the MTT assay, and inverted fluorescence microscopy were employed to determine the effects of MgO NPs on C. albicans adhesion, two-phase morphological transformation, biofilm biomass, and metabolic activity. The time-kill curve showed that MgO NPs had fungicidal and antifungal activity against C. albicans in a time- and concentration-dependent manner. Semi-quantitative crystal violet staining and MTT assays showed that MgO NPs significantly inhibited C. albicans biofilm formation and metabolic activity, and the difference was statistically significant (p < 0.001). Inverted fluorescence microscopy showed that MgO NPs could inhibit the formation of C. albicans biofilm hyphae. Adhesion experiments showed that MgO NPs significantly inhibited the initial adhesion of C. albicans (p < 0.001). This study demonstrates that MgO NPs can effectively inhibit the growth, initial adhesion, two-phase morphological transformation, and biofilm formation of C. albicans and is an antifungal candidate.
引用
收藏
页码：485 / 494
页数：9
相关论文
共 50 条
[31]   β-lapachone and α-nor-lapachone modulate Candida albicans viability and virulence factors [J].
Moraes, D. C. ;
Curvelo, J. A. R. ;
Anjos, C. A. ;
Moura, K. C. G. ;
Pinto, M. C. F. R. ;
Portela, M. B. ;
Soares, R. M. A. .
JOURNAL DE MYCOLOGIE MEDICALE, 2018, 28 (02) :314-319
[32]   Beta vulgaris Assisted Fabrication of Novel Ag-Cu Bimetallic Nanoparticles for Growth Inhibition and Virulence in Candida albicans [J].
Kamli, Majid Rasool ;
Malik, Maqsood Ahmad ;
Lone, Shabir Ahmad ;
Sabir, Jamal S. M. ;
Mattar, Ehab H. ;
Ahmad, Aijaz .
PHARMACEUTICS, 2021, 13 (11)
[33]   Antifungal Activity of Condensed Tannins from Stryphnodendron adstringens: Effect on Candida tropicalis Growth and Adhesion Properties [J].
Morey, Alexandre T. ;
de Souza, Felipe C. ;
Santos, Jussevania P. ;
Pereira, Caibe A. ;
Cardoso, Juscelio D. ;
de Almeida, Ricardo S. C. ;
Costa, Marco A. ;
de Mello, Joao C. P. ;
Nakamura, Celso V. ;
Pinge-Filho, Phileno ;
Yamauchi, Lucy M. ;
Yamada-Ogatta, Sueli F. .
CURRENT PHARMACEUTICAL BIOTECHNOLOGY, 2016, 17 (04) :365-375
[34]   Antifungal Activity of a Hydroethanolic Extract From Astronium urundeuva Leaves Against Candida albicans and Candida glabrata [J].
Bonifacio, Bruna Vidal ;
Machado Vila, Taissa Vieira ;
Masiero, Isadora Fantacini ;
da Silva, Patricia Bento ;
da Silva, Isabel Cristiane ;
Lopes, Erica de Oliveira ;
dos Santos Ramos, Matheus Aparecido ;
de Souza, Leonardo Perez ;
Vilegas, Wagner ;
Pavan, Fernando Rogerio ;
Chorilli, Marlus ;
Lopez-Ribot, Jose Luis ;
Bauab, Tais Maria .
FRONTIERS IN MICROBIOLOGY, 2019, 10
[35]   Diphenyl diselenide suppresses key virulence factors of Candida krusei, a neglected fungal pathogen [J].
Marques da Silva, Bruna Graziele ;
Pinto, Ana Paula ;
da Silva Passos, Juliene Cristina ;
Teixeira da Rocha, Joao Batista ;
Alberto-Silva, Carlos ;
Costa, Maricilia Silva .
BIOFOULING, 2022, 38 (05) :427-440
[36]   Virulence Factors and Antifungal Susceptibility in Candida Species Isolated from Dermatomycosis Patients [J].
Mello, Victor Galvao ;
Escudeiro, Heloisa ;
Weckwerth, Ana Carolina Villas Boas ;
Andrade, Maria Izilda ;
Fusaro, Ana Elisa ;
de Moraes, Eloise Brasil ;
Ruiz, Luciana da Silva ;
Baptista, Ida Maria Foschiani Dias .
MYCOPATHOLOGIA, 2021, 186 (01) :71-80
[37]   Effect of Candida albicans' supernatant on biofilm formation and virulence factors of Pseudomonas aeruginosa through las/rhl System [J].
Zhang, Ke ;
Huang, Yingying ;
Jiang, Yuting ;
Liu, Tangjuan ;
Kong, Jinliang ;
Cai, Shuangqi ;
Wen, Zhongwei ;
Chen, Yiqiang .
BMC MICROBIOLOGY, 2025, 25 (01)
[38]   Antifungal effect of a new photosensitizer derived from BODIPY on Candida albicans biofilms [J].
Wang, Mengran ;
Gu, Kedan ;
Ding, Wenxin ;
Wan, Miyang ;
Zhao, Weili ;
Shi, Hang ;
Li, Jiyang .
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY, 2022, 39
[39]   Physiological and transcriptional profiling of surfactin exerted antifungal effect against Candida albicans [J].
Jakab, Agnes ;
Kovacs, Fruzsina ;
Balla, Noemi ;
Toth, Zoltan ;
Ragyak, Agota ;
Sajtos, Zsofi ;
Csillag, Kinga ;
Nagy-Koteles, Csaba ;
Nemes, Daniel ;
Bacskay, Ildiko ;
Pocsi, Istvan ;
Majoros, Laszlo ;
Kovacs, Akos T. ;
Kovacs, Renato .
BIOMEDICINE & PHARMACOTHERAPY, 2022, 152
[40]   Antifungal effect of photodynamic therapy mediated by curcumin on Candida albicans biofilms in vitro [J].
Ma, Jing ;
Shi, Hang ;
Sun, Hongying ;
Li, Jiyang ;
Bai, Yu .
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY, 2019, 27 :280-287
12345