Development of an enzyme-linked immunosorbent assay for atrazine monitoring in water samples

被引：0
作者：
Diana L. D. Lima
Rudolf J. Schneider
Valdemar I. Esteves
机构：
[1] University of Aveiro,CESAM & Department of Chemistry
[2] Superior School of Health Technology of Coimbra,Department of Complementary Sciences
[3] BAM Federal Institute for Materials Research and Testing,Department of Analytical Chemistry, Reference Materials
来源：
Environmental Science and Pollution Research | 2013年 / 20卷
关键词：
Immunoassay; Atrazine; Surface waters; Wastewater treatment plants; Matrix effects;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The implementation of the Water Framework Directive (2000/60/EC) requires the establishment of monitoring programs. However, conventional procedures for sample preparation prior to chromatographic analysis are rather expensive and time consuming, being the development of cost-effective and easy tool a necessity. The aim of this work was to develop an enzyme-linked immunosorbent assay (ELISA) able to determine atrazine in water samples. Matrix effects evaluation showed that the increase of humic acid (HA) concentration leads to flattened calibration curves and to the loss of the sigmoidal shape. However, such interference was overcome, by the presence of an environmental sample buffer, incubated together with the samples. Recoveries from 88.5 to 119.2 % were obtained in the presence of HA concentrations up to 20 mg L−1. An analytical range from 0.003 to 1 μg L−1 was obtained, and atrazine was detected in a sewage treatment plant with concentrations ranging from 14 to 52 ng L−1.
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页码：3157 / 3164
页数：7
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