Computational design and characterization of a temperature-sensitive plasmid replicon for gram positive thermophiles

[1] Demain AL(2005)Cellulase,clostridia, and ethanol Microbiol Mol Biol Rev 69 124-154

[2] Newcomb M(2010)Deletion of the Cel48S cellulase fromClostridium thermocellum Proc Natl Acad Sci USA 107 17727-17732

[3] Wu JHD(2010)Development ofpyrF-based genetic system for targeted gene deletion inClostridium thermocellumand creation of aptamutant Appl Environ Microbiol 76 6591-6599

[4] Olson DG(2011)Marker removal system forThermoanaerobacterium saccharolyticumand development of a markerless ethanologen Appl Environ Microbiol 77 2534-2536

[5] Tripathi SA(2010)Characterization of a cryptic plasmid from Pseudomonas sp. and utilization of its temperature-sensitive derivatives for genetic manipulation Plasmid 64 110-117

[6] Giannone RJ(2008)Development of a markerless gene knock-out system forMannheimia succiniciproducensusing a temperature-sensitive plasmid FEMS Microbiol Lett 278 78-85

[7] Lo J(2005)Generation and molecular characterization of new temperature-sensitive plasmids intended for genetic engineering ofPasteurellaceae Appl Environ Microbiol 71 7187-7195

[8] Caiazza NC(2002)A high temperature-sensitive mutant of Synechococcus sp PCC 7002 with modifications in the endogenous plasmid, pAQ1 Plant Cell Physiol 43 217-223

[9] Hogsett DA(2000)A set of temperature sensitive-replication/-segregation and temperature resistant plasmid vectors with different copy numbers and in an isogenic background (chloramphenicol, kanamycin, lacZ, repA, par, polA) Gene 241 185-191

[10] Hettich RL(199)Isolation of a derivative ofEscherichia coli Enterococcus faecalisshuttle vector pAM401 temperature sensitive for maintenance inE. faecalisand its use in evaluating the mechanism of pAD1par-dependent plasmid stabilization Plasmid 40 225-232

[1] Demain AL(2005)Cellulase,clostridia, and ethanol Microbiol Mol Biol Rev 69 124-154

[2] Newcomb M(2010)Deletion of the Cel48S cellulase fromClostridium thermocellum Proc Natl Acad Sci USA 107 17727-17732

[3] Wu JHD(2010)Development ofpyrF-based genetic system for targeted gene deletion inClostridium thermocellumand creation of aptamutant Appl Environ Microbiol 76 6591-6599

[4] Olson DG(2011)Marker removal system forThermoanaerobacterium saccharolyticumand development of a markerless ethanologen Appl Environ Microbiol 77 2534-2536

[5] Tripathi SA(2010)Characterization of a cryptic plasmid from Pseudomonas sp. and utilization of its temperature-sensitive derivatives for genetic manipulation Plasmid 64 110-117

[6] Giannone RJ(2008)Development of a markerless gene knock-out system forMannheimia succiniciproducensusing a temperature-sensitive plasmid FEMS Microbiol Lett 278 78-85

[7] Lo J(2005)Generation and molecular characterization of new temperature-sensitive plasmids intended for genetic engineering ofPasteurellaceae Appl Environ Microbiol 71 7187-7195

[8] Caiazza NC(2002)A high temperature-sensitive mutant of Synechococcus sp PCC 7002 with modifications in the endogenous plasmid, pAQ1 Plant Cell Physiol 43 217-223

[9] Hogsett DA(2000)A set of temperature sensitive-replication/-segregation and temperature resistant plasmid vectors with different copy numbers and in an isogenic background (chloramphenicol, kanamycin, lacZ, repA, par, polA) Gene 241 185-191

[10] Hettich RL(199)Isolation of a derivative ofEscherichia coli Enterococcus faecalisshuttle vector pAM401 temperature sensitive for maintenance inE. faecalisand its use in evaluating the mechanism of pAD1par-dependent plasmid stabilization Plasmid 40 225-232