Comparative proteomic analysis of outer membrane vesicles from Brucella suis, Brucella ovis, Brucella canis and Brucella neotomae

被引：0

作者：

María del Socorro Ruiz-Palma

Eric Daniel Avila-Calderón

Ma. Guadalupe Aguilera-Arreola

Ahidé López-Merino

Enrico A. Ruiz

María del Rosario Morales-García

Edgar Oliver López-Villegas

Zulema Gomez-Lunar

Beatriz Arellano-Reynoso

Araceli Contreras-Rodríguez

机构：

[1] Escuela Nacional de Ciencias Biológicas (ENCB),Departamento de Microbiología

[2] Instituto Politécnico Nacional (IPN),División Químico Biológicas

[3] Universidad Tecnológica de Tecámac,Departamento de Biología Celular, Centro de Investigación y de Estudios Avanzados

[4] Instituto Politécnico Nacional,Departamento de Zoología

[5] CINVESTAV-IPN,Departamento de Investigación

[6] Avenida Instituto Politécnico Nacional 2508,Departamento de Investigación, Central de Microscopía

[7] Escuela Nacional de Ciencias Biológicas (ENCB),Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia

[8] Instituto Politécnico Nacional (IPN),undefined

[9] CICATA Querétaro,undefined

[10] Instituto Politécnico Nacional (IPN),undefined

[11] Escuela Nacional de Ciencias Biológicas (ENCB),undefined

[12] Instituto Politécnico Nacional (IPN),undefined

[13] Universidad Nacional Autónoma de México (UNAM),undefined

来源：

Archives of Microbiology | 2021年 / 203卷

关键词：

Outer membrane vesicles; Brucellosis; Bacterial vesicles; Acellular vaccines;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Gram-negative bacteria release nanovesicles, called outer membrane vesicles (OMVs), from their outer membrane. Proteomics has been used to determine their composition. OMVs contain proteins able to elicit an immune response, so they have been proposed as a model to develop acellular vaccines. In this study, OMVs of Brucella suis, B. ovis, B. canis, and B. neotomae were purified and analyzed by SDS-PAGE, transmission electron microscopy and liquid chromatography coupled to mass spectrometry to determine the pan-proteome of these vesicles. In addition, antigenic proteins were detected by western blot with anti-Brucella sera. The in silico analysis of the pan-proteome revealed many homologous proteins, such as Omp16, Omp25, Omp31, SodC, Omp2a, and BhuA. Proteins contained in the vesicles from different Brucella species were detected by anti-Brucella sera. The occurrence of previously described immunogenic proteins derived from OMVs supports the use of these vesicles as candidates to be evaluated as an acellular brucellosis vaccine.

引用

页码：1611 / 1626

页数：15

共 355 条

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