TDZ-induced direct shoot organogenesis and somatic embryogenesis on cotyledonary node explants of lentil (Lens culinaris Medik.)

被引：27

作者：

Chhabra G. ^{[1
]}

Chaudhary D. ^{[1
]}

Varma M. ^{[1
]}

Sainger M. ^{[1
]}

Jaiwal P.K. ^{[1
]}

机构：

[1] Advanced Centre for Biotechnology, M.D. University

来源：

Physiology and Molecular Biology of Plants | 2008年 / 14卷 / 4期

关键词：

Lens culinaris; Organogenesis; Somatic embryogenesis; Thidiazuron;

D O I：

10.1007/s12298-008-0033-z

中图分类号：

学科分类号：

摘要：

An efficient and simple procedure for inducing high frequency direct shoot organogenesis and somatic embryogenesis in lentil from cotyledonary node explants (without both the cotyledons) in response to TDZ alone is reported. TDZ at concentration lower than 2.0 μM induced shoot organogenesis whereas at higher concentration (2.5-15 μM) it caused a shift in regeneration from shoot organogenesis to somatic embryogenesis. The cotyledonary node and seedling cultures developed only shoots even at high concentrations of BAP and TDZ, respectively. TDZ at 0.5 and 5.0 μM was found to be optimal for inducing an average of 4-5 shoots per cotyledonary node in 93 % of the cultures and 55 somatic embryos in 68 % of the cultures, respectively. The somatic embryos were germinated when transferred to lower TDZ concentration (0.5-1.0 μM). The shoots were rooted on MS basal medium containing 2.5 μM IBA. The plantlets were obtained within 8 weeks from initiation of culture and were morphologically similar to seed-raised plants. The possible role of stress in thidiazuron induced somatic embryogenesis is discussed. © 2008 Prof. H.S. Srivastava Foundation for Science and Society.

引用

页码：347 / 353

页数：6

共 35 条

[1]

Amutha S., Muruganantham, Ganapathi A., Thidiazuron induced high frequency axillary and adventitious shoot regeneration in Vigna radiata L. Wilczek, In Vitro Cell Dev Biol Plant, 42, pp. 26-30, (2006)

[2]

Bajaj Y.P.S., Dhanju M.S., Regeneration of plants from apical meristem tips of some legumes, Curr Sci, 84, pp. 906-907, (1979)

[3]

Bates S., Preece J.E., Navarrete N.E., TDZ stimulates shoots organogenesis in white ash (Fraximus americana L.), Plant Cell Tiss. Org. Cult., 31, pp. 21-29, (1992)

[4]

Brunning J.L., Kintz B.L., (1977)

[5]

Chaudhary D., Madanpotra S., Jaiwal R., Saini R., Kumar P.A., Jaiwal P.K., Agrobacterium tumefaciens-mediated high frequency genetic transformation of Indian cowpea (Vigna unguiculata L. Walp.), Plant Sci., 172, pp. 692-700, (2006)

[6]

FAOSTAT Report, (2007)

[7]

Gairi A., Rashid A., Direct differentiation of somatic embryos on different regions of intact seedlings of Azadirachta in response to thidiazuron, J. Plant Physiol., 161, pp. 1073-1077, (2004)

[8]

Gill R., Saxena P.K., Direct somatic embryogenesis and regeneration of plants from seedling explants of peanut (Arachis hypogaea): Promotive role of TDZ, Can. J. Bot., 70, pp. 1186-1192, (1992)

[9]

Gulati A., Schreyer P., Mc Hughen A., Regeneration and micro-grafting of lentil shoots, In Vitro Cell. Dev. Biol. Plant, 37, pp. 798-802, (2001)

[10]

Gulati A., Mc Hughen A., (2003)

← 1 2 3 4 →