Effects of aspirin and clopidogrel on neural stem cells

被引:0
作者
Mina Hwang
Hyun-Hee Park
Hojin Choi
Kyu-Yong Lee
Young Joo Lee
Seong-Ho Koh
机构
[1] Hanyang University College of Medicine,Department of Neurology
[2] ,Department of Translational Medicine, Hanyang University Graduate School of Biomedical Science and Engineering
[3] Hanyang University,undefined
来源
Cell Biology and Toxicology | 2018年 / 34卷
关键词
Neural stem cells; Aspirin; Clopidogrel bisulfate; Clopidogrel resinate; Proliferation; Viability;
D O I
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中图分类号
学科分类号
摘要
Cerebral infarction causes severe morbidity and mortality. Most patients with cerebral infarction should take antiplatelet drugs daily, so the effects of those drugs on the regeneration of the brain need to be investigated. Aspirin and clopidogrel are the most widely used antiplatelet drugs for the prevention of ischemic stroke. We investigated the effects of aspirin and clopidogrel on neural stem cells (NSCs). NSCs were dissociated from fetal rat cortex and cultured with basic fibroblast growth factor and N2 medium. To measure the effects of aspirin and clopidogrel on NSCs, NSCs were treated with several concentrations of aspirin, clopidogrel bisulfate, and clopidogrel resinate for 24 h. After the treatment, we measured cell viability by cell counting kit-8, MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay, trypan blue staining, flow cytometry, and lactate dehydrogenase assay. To evaluate their effects on NSC proliferation, we performed BrdU cell proliferation assay and colony-forming unit assay. We compared the intracellular protein level in the NSCs treated with aspirin and two types of clopidogrel, by proteomics analysis. Various viability tests showed that clopidogrel resinate and clopidogrel bisulfate did not affect the viability and proliferation of NSCs whereas aspirin decreased them even at low concentrations which are clinically relevant. Moreover, through the proteomics, it was confirmed that the toxicity of aspirin to NSCs might be associated with the alteration of several intracellular proteins. Taken together, these results suggest that clopidogrel resinate and clopidogrel bisulfate are safe but aspirin could be toxic to NSCs. Therefore, when these antiplatelet agents are prescribed over the long-term, the finding that aspirin could be toxic to NSCs should be considered.
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页码:219 / 232
页数:13
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