Assembly of phospholipid nanodiscs of controlled size for structural studies of membrane proteins by NMR

被引：0
|
作者：
Franz Hagn
Mahmoud L Nasr
Gerhard Wagner
机构：
[1] Technical University of Munich,Center for Integrated Protein Science Munich (CIPSM) at the Department of Chemistry and Institute for Advanced Study
[2] Helmholtz Center Munich,Department of Biological Chemistry and Molecular Pharmacology
[3] Institute of Structural Biology,undefined
[4] Harvard Medical School,undefined
来源：
Nature Protocols | 2018年 / 13卷
关键词：
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The applications of solution-state NMR of membrane proteins are often limited by difficulty in finding a suitable membrane mimetic of tailored size that shows native-like membrane properties and provides long-term stability. This protocol describes how to assemble phospholipid nanodiscs and incorporate membrane proteins for NMR-structural studies.
引用
收藏
页码：79 / 98
页数：19
相关论文
共 50 条
  • [31] NMR structural studies of the FXYD membrane proteins in lipid environments
    Crowell, KJ
    Franzin, CM
    Kohay, A
    Marassi, FM
    FASEB JOURNAL, 2003, 17 (05): : A1322 - A1322
  • [32] NMR structural studies of the FXYD family of proteins in membrane environments
    Franzin, CM
    Thai, K
    Yu, JG
    Marassi, FM
    BIOPHYSICAL JOURNAL, 2005, 88 (01) : 222A - 222A
  • [33] The Advanced Properties of Circularized MSP Nanodiscs Facilitate High-resolution NMR Studies of Membrane Proteins
    Daniilidis, Melina
    Brandl, Matthias J.
    Hagn, Franz
    JOURNAL OF MOLECULAR BIOLOGY, 2022, 434 (24)
  • [34] Choosing membrane mimetics for NMR structural studies of transmembrane proteins
    Warschawski, Dror E.
    Arnold, Alexandre A.
    Beaugrand, Maiwenn
    Gravel, Andree
    Chartrand, Etienne
    Marcotte, Isabelle
    BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2011, 1808 (08): : 1957 - 1974
  • [35] Structural studies of membrane proteins using MAS-NMR.
    Cocco, MJ
    Javadpour, M
    Shieh, T
    Song, DK
    Smith, SO
    BIOPHYSICAL JOURNAL, 1997, 72 (02) : TU326 - TU326
  • [36] Assembly of deuterated phospholipid nanodiscs for Small-Angle Neutron Scattering studies
    Leite, Wellington
    Zhang, Qiu
    Qian, Shuo
    O'Neill, Hugh
    ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES, 2022, 78 : A187 - A187
  • [37] Phospholipid Bilayer Nanodiscs: A Powerful Tool to Study the Structural Organization and Biochemical Reactivity of Proteins in Membrane-like Environments
    Hernandez-Rocamora, Victor M.
    Garcia-Montanes, Concepcion
    Rivas, German
    CURRENT TOPICS IN MEDICINAL CHEMISTRY, 2014, 14 (23) : 2637 - 2646
  • [38] Hydration-optimized oriented phospholipid bilayer samples for solid-state NMR structural studies of membrane proteins
    Marassi, FM
    Crowell, KJ
    JOURNAL OF MAGNETIC RESONANCE, 2003, 161 (01) : 64 - 69
  • [39] Conformational Analysis of Membrane Proteins in Phospholipid Bilayer Nanodiscs by Hydrogen Exchange Mass Spectrometry
    Hebling, Christine M.
    Morgan, Christopher R.
    Stafford, Darrel W.
    Jorgenson, James W.
    Rand, Kasper D.
    Engen, John R.
    ANALYTICAL CHEMISTRY, 2010, 82 (13) : 5415 - 5419
  • [40] NMR studies of membrane proteins
    Kaptein, Rob
    Wagner, Gerhard
    JOURNAL OF BIOMOLECULAR NMR, 2015, 61 (3-4) : 181 - 184
12345