Assembly of phospholipid nanodiscs of controlled size for structural studies of membrane proteins by NMR

被引：0
|
作者：
Franz Hagn
Mahmoud L Nasr
Gerhard Wagner
机构：
[1] Technical University of Munich,Center for Integrated Protein Science Munich (CIPSM) at the Department of Chemistry and Institute for Advanced Study
[2] Helmholtz Center Munich,Department of Biological Chemistry and Molecular Pharmacology
[3] Institute of Structural Biology,undefined
[4] Harvard Medical School,undefined
来源：
Nature Protocols | 2018年 / 13卷
关键词：
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The applications of solution-state NMR of membrane proteins are often limited by difficulty in finding a suitable membrane mimetic of tailored size that shows native-like membrane properties and provides long-term stability. This protocol describes how to assemble phospholipid nanodiscs and incorporate membrane proteins for NMR-structural studies.
引用
收藏
页码：79 / 98
页数：19
相关论文
共 50 条
  • [1] Assembly of phospholipid nanodiscs of controlled size for structural studies of membrane proteins by NMR
    Hagn, Franz
    Nasr, Mahmoud L.
    Wagner, Gerhard
    NATURE PROTOCOLS, 2018, 13 (01) : 79 - 98
  • [2] Nanodiscs for structural and functional studies of membrane proteins
    Denisov, Ilia G.
    Sligar, Stephen G.
    NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2016, 23 (06) : 481 - 486
  • [3] Nanodiscs for structural and functional studies of membrane proteins
    Ilia G Denisov
    Stephen G Sligar
    Nature Structural & Molecular Biology, 2016, 23 : 481 - 486
  • [4] Production and Structural Analysis of Membrane-Anchored Proteins in Phospholipid Nanodiscs
    Raltchev, Kolio
    Pipercevic, Joka
    Hagn, Franz
    CHEMISTRY-A EUROPEAN JOURNAL, 2018, 24 (21) : 5493 - 5499
  • [5] Directed Self-Assembly of Monodisperse Phospholipid Bilayer Nanodiscs with Controlled Size
    Sligar, S.G. (s-sligar@uiuc.edu), 1600, American Chemical Society (126):
  • [6] Directed self-assembly of monodisperse phospholipid bilayer nanodiscs with controlled size
    Denisov, IG
    Grinkova, YV
    Lazarides, AA
    Sligar, SG
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (11) : 3477 - 3487
  • [7] Using phospholipid nanodiscs as a platform for Small-Angle Scattering based structural studies of membrane proteins
    Arleth, Lise
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2013, 245
  • [8] RECONSTITUTION OF MEMBRANE PROTEINS IN PHOSPHOLIPID BILAYER NANODISCS
    Ritchie, T. K.
    Grinkova, Y. V.
    Bayburt, T. H.
    Denisov, I. G.
    Zolnerciks, J. K.
    Atkins, W. M.
    Sligar, S. G.
    METHODS IN ENZYMOLOGY; LIPOSOMES, PT F, 2009, 464 : 211 - 231
  • [9] Solution NMR Spectroscopy and Protein Interaction Studies of Membrane Proteins in Nanodiscs
    Glueck, Julian M.
    Wittlich, Marc
    Feuerstein, Sophie
    Willbold, Dieter
    Koenig, Bernd W.
    BIOPHYSICAL JOURNAL, 2011, 100 (03) : 551 - 552
  • [10] Lipid Nanodiscs for High-Resolution NMR Studies of Membrane Proteins
    Gunsel, Umut
    Hagn, Franz
    CHEMICAL REVIEWS, 2022, 122 (10) : 9395 - 9421
12345