Detection of Protein Kinase A and C Target Proteins in Rat Brain Mitochondria

被引:2
作者
Krestinina O.V. [1 ]
Odinokova I.V. [1 ]
Baburina Y.L. [1 ]
Azarashvili T.S. [1 ]
机构
[1] Institute of Theoretical and Experimental Biophysics, Russian Academy of Science, Pushchino, Moscow oblast
关键词
brain mitochondria; permeability transition pore; protein kinase A; protein kinase C;
D O I
10.1134/S1990747818010087
中图分类号
学科分类号
摘要
Phosphorylation of some membrane-bound proteins in the mitochondria of rat liver and brain is regulated by Ca2+ and cAMP acting as secondary messengers. These proteins are the main myelin components: 46 kDa 2′,3′-cyclic-nucleotide 3′-phosphodiesterase (CNP) and two isoforms of the myelin basic protein (MBP) with molecular weights of 17 and 21.5 kDa, which we have identified previously and found outside myelin in rat brain mitochondria. The phosphorylation level of CNP and both MBP isoforms increases when the mitochondrial permeability transition pore (mPTP) is opened. It is known that protein kinases A and C in heart mitochondria are directly bound to mPTP regulator proteins and are able to modulate the pore function. It is shown in this study that the inhibitors of protein kinases A (H-89) and C (staurosporin, Go 6976, and GF 109203 X) decrease the phosphorylation level of CNP and two MBP isoforms allowing us to assume that they are the targets of the signaling protein kinases A and C. © 2018, Pleiades Publishing, Ltd.
引用
收藏
页码:70 / 73
页数:3
相关论文
共 50 条
[41]   Protein kinase Cε interacts with and inhibits the permeability transition pore in cardiac mitochondria [J].
Baines, CP ;
Song, CX ;
Zheng, YT ;
Wang, GW ;
Zhang, J ;
Wang, OL ;
Guo, Y ;
Bolli, R ;
Cardwell, EM ;
Ping, PP .
CIRCULATION RESEARCH, 2003, 92 (08) :873-880
[42]   INCREASED PHOSPHORYLATION OF NUCLEAR SUBSTRATES FOR RAT-BRAIN PROTEIN-KINASE-C IN REGENERATING RAT-LIVER NUCLEI [J].
MAZZONI, M ;
CARINI, C ;
MATTEUCCI, A ;
MARTELLI, AM ;
BERTAGNOLO, V ;
PREVIATI, M ;
RANA, R ;
CATALDI, A ;
CAPITANI, S .
CELLULAR SIGNALLING, 1992, 4 (03) :313-319
[43]   EFFECT OF HYPOTHYROIDISM AND THYROID-HORMONE REPLACEMENT ON THE LEVEL OF PROTEIN-KINASE-C AND PROTEIN KINASE-A IN RAT-LIVER [J].
MEIER, CA ;
FABBRO, D ;
MEYHACK, I ;
HEMMINGS, B ;
OLBRECHT, U ;
JAKOB, A ;
WALTER, P .
FEBS LETTERS, 1991, 282 (02) :397-400
[44]   A novel protein kinase C target site in protein kinase D is phosphorylated in response to signals for cardiac hypertrophy [J].
Phan, Dillon ;
Stratton, Matthew S. ;
Huynh, Q. Khai ;
McKinsey, Timothy A. .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2011, 411 (02) :335-341
[45]   Dinucleotide receptor modulation by protein kinases (protein kinases A and C) and protein phosphatases in rat brain synaptic terminals [J].
Pintor, J ;
Gualix, J ;
MirasPortugal, MT .
JOURNAL OF NEUROCHEMISTRY, 1997, 68 (06) :2552-2557
[46]   Protein kinase C: A potential therapeutic target for endothelial dysfunction in diabetes [J].
Xiao, Qian ;
Wang, Dan ;
Li, Danyang ;
Huang, Jing ;
Ma, Feifei ;
Zhang, Haocheng ;
Sheng, Yingda ;
Zhang, Caimei ;
Ha, Xiaoqin .
JOURNAL OF DIABETES AND ITS COMPLICATIONS, 2023, 37 (09)
[47]   Protein kinase Cα is a calpain target in cultured embryonic muscle cells [J].
Aragon, B ;
Poussard, S ;
Dulong, S ;
Touyarot, K ;
Dargelos, E ;
Brustis, JJ ;
Levieux, D ;
Ducastaing, A ;
Cottin, P .
MOLECULAR AND CELLULAR BIOCHEMISTRY, 2002, 231 (1-2) :97-106
[48]   Protein kinase Cα is a calpain target in cultured embryonic muscle cells [J].
B. Aragon ;
S. Poussard ;
S. Dulong ;
K. Touyarot ;
E. Dargelos ;
J.J. Brustis ;
D. Levieux ;
A. Ducastaing ;
P. Cottin .
Molecular and Cellular Biochemistry, 2002, 231 :97-106
[49]   Protein kinase C in the postmortem brain of teenage suicide victims [J].
Pandey, GN ;
Dwivedi, Y ;
Pandey, SC ;
Conley, RR ;
Roberts, RC ;
Tamminga, CA .
NEUROSCIENCE LETTERS, 1997, 228 (02) :111-114
[50]   PROTEIN-KINASE-C IN RAT-BRAIN CORTEX AND HIPPOCAMPUS - EFFECT OF REPEATED ADMINISTRATION OF FLUOXETINE AND DESIPRAMINE [J].
MANN, CD ;
VU, TB ;
HRDINA, PD .
BRITISH JOURNAL OF PHARMACOLOGY, 1995, 115 (04) :595-600