Evaluation of methods of synchronization of cell division in yeast Saccharomyces cerevisiae

被引：2
|
作者：
Zhuk A.S. [1 ,2 ]
Stepchenkova E.I. [1 ,2 ]
Pavlov Y.I. [3 ]
Inge-Vechtomov S.G. [1 ,2 ]
机构：
[1] St. Petersburg Branch of N.I. Vavilov Institute of General Genetics, Russian Academy of Sciences, St. Petersburg
[2] St. Petersburg State University, St. Petersburg
[3] University of Nebraska Medical Center, Omaha
来源：
Cell and Tissue Biology | 2017年 / 11卷 / 2期
关键词：
alpha-factor; cdc28-4; flow cytometry; hydroxyurea; nocodazole; synchronize of cell division; yeast Saccharomyces cerevisiae;
D O I：
10.1134/S1990519X17020110
中图分类号：
学科分类号：
摘要：
Synchronization of cell division in yeast cultures of Saccharomyces cerevisiae is widely used in studies on regulation of eukaryotic gene expression and biochemical processes at different stages of the cell cycle. In this study, we compared the efficacy of modern widely used methodologies to achieve and assess the degree of synchronization of cell division in yeast. Based on the literature and our own data, we propose practical recommendations for synchronization of cell divisions in S. cerevisiae using chemical reagents (alpha-factor, hydroxyurea, nocodazole), and a genetic cell-cycle block (temperature-sensitive mutation cdc28-4). © 2017, Pleiades Publishing, Ltd.
引用
收藏
页码：111 / 122
页数：11
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