Phylogenetic analysis of the hemagglutinin gene of influenza A(H1N1)pdm09 and A(H3N2) virus isolates from Haryana, India

被引：6

作者：

Sharma V. ^{[1
]}

Sharma M. ^{[2
]}

Dhull D. ^{[1
]}

Kaushik S. ^{[3
]}

机构：

[1] Centre for Biotechnology, Maharshi Dayanand University, Rohtak

[2] Department of Biotechnology, Shoolini Institute of Life Sciences and Business Management, Solan

[3] Department of Genetics, Maharshi Dayanand University, Rohtak

来源：

VirusDisease | 2019年 / 30卷 / 3期

关键词：

A(H1N1)pdm09; H3N2; Hemagglutinin; Influenza; Phylogenetic analysis; Real-time RT-PCR;

D O I：

10.1007/s13337-019-00532-7

中图分类号：

学科分类号：

摘要：

Influenza A viruses are highly adaptable and are the main pathogen behind winter time morbidity. The present study reports the molecular and phylogenetic characterization of A(H1N1)pdm09 and H3N2 isolates from Haryana, India during 2015 influenza outbreak. A total of 144 nasopharyngeal samples were collected from Post Graduate Institute of Medical Sciences, Rohtak, Haryana, India form September 2014 to February 2016. The samples were screened for influenza A subtypes; A(H1N1)pdm09 and H3N2 by using real-time RT-PCR. Virus isolation and hemagglutinin gene sequencing studies were performed for selected positive samples. Out of 24 (16.6%) Influenza A positive samples, 13 (54.2%) and 11 (45.8%) were subtyped into A(H1N1)pdm09 and H3N2, respectively by real-time RT-PCR. Genetic analysis of A(H1N1)pdm09 isolates revealed the presence of key mutations (P100S, S202T and S220T) in HA gene as compare to reference strain A/California/07/2009 and these isolates were grouped in clade 6B.1 and 6B.2. All A(H3N2) isolates were clustered in clade 3C.2a and revealed specific amino acid substitutions of N161S and P214S in their HA genes in comparison to the reference strain A/Texas/50/2012. The HA gene sequences of all isolates showed 97–98% of nucleotide sequence similarity with their respective reference strains. Influenza A(H1N1)pdm09 and H3N2 isolates were drifted significantly from their respective vaccines strains of 2015–2016 and were more closely related to recommended vaccine strains for flu season 2017–2018. The study supports the need of routine influenza surveillance and continuous monitoring of the genetic changes in the major antigenic sites of these viruses. © 2019, Indian Virological Society.

引用

页码：336 / 343

页数：7

共 29 条

[21]

Sharma V., Chaudhry D., Kaushik S., Evaluation of clinical applicability of reverse transcription-loop-mediated isothermal amplification assay for detection and subtyping of Influenza A viruses, J Virol Methods, 253, pp. 18-25, (2018)

[22]

Shaw M.L., Palese P., Orthomyxoviridae, Fields virology, pp. 1151-1185, (2013)

[23]

Skehel J.J., Stevens D.J., Daniels R.S., Douglas A.R., Knossow M., Wilson I.A., Et al., A carbohydrate side chain on hemagglutinins of Hong Kong influenza viruses inhibits recognition by a monoclonal antibody, Proc Natl Acad Sci USA, 81, pp. 1779-1783, (1984)

[24]

Suzuki Y., Ito T., Suzuki T., Holland R.E., Chambers T.M., Kiso M., Et al., Sialic acid species as a determinant of the host range of influenza A viruses, J Virol, 74, pp. 11825-11831, (2000)

[25]

Tamura K., Stecher G., Peterson D., Filipski A., Kumar S., MEGA6: molecular evolutionary genetics analysis version 6.0, Mol Biol Evol, 30, pp. 2725-2729, (2013)

[26]

Tumpey T.M., Garcia-Sastre A., Taubenberger J.K., Palese P., Swayne D.E., Basler C.F., Pathogenicity and immunogenicity of influenza viruses with genes from the 1918 pandemic virus, Proc Natl Acad Sci USA, 101, pp. 3166-3171, (2004)

[27]

Webster R.G., Govorkova E.A., Continuing challenges in influenza, Ann N Y Acad Sci, 1323, pp. 115-139, (2014)

[28]

Wiley D.C., Wilson I.A., Skehel J.J., Structural identification of the antibody-binding sites of Hong Kong influenza haemagglutinin and their involvement in antigenic variation, Nature, 289, pp. 373-378, (1981)

[29]

Zhong J., Liang L., Huang P., Zhu X., Zou L., Yu S., Et al., Genetic mutations in influenza H3N2 viruses from a 2012 epidemic in Southern China, Virol J, 10, (2013)

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