Cell Culture Test System for Express Analysis of Cytotoxic and Growth-Stimulating Effects of Bioactive Compounds

被引：0
作者：
V. G. Chernikov
S. M. Terekhov
T. B. Krokhina
S. S. Shishkin
T. D. Smirnova
N. V. Adnoral
L. B. Rebrov
Yu. I. Denisov-Nikol'skii
B. A. Bykov
机构：
[1] Russian Academy of Medical Sciences,Medical Genetic Research Center
[2] Research Center of Biomedical Technologies,undefined
来源：
Bulletin of Experimental Biology and Medicine | 2001年 / 131卷
关键词：
human cell cultures; biotest system; cytotoxicity; growth-stimulating effects; biologically active compounds;
D O I：
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摘要：
Cultures of human and mammalian cells presenting 4 types of differentiation (normal human fibroblasts and myoblasts, human and Syrian hamster hepatoma cells, and mouse/mouse hybridoma cells) were used in a panel biotest system. This system allowed to evaluate the cytotoxic and stimulatory effect of bioactive compounds by determining the dose-effect relationships and some quantitative parameters including LD50. Examination of some biolactive compounds of different nature (sangviritrin, escin, deltostim, cycloheximide, dexamethasone) confirmed high efficacy of this biotest system.
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页码：573 / 575
页数：2
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