Isolation and characterization of 23 novel polymorphic microsatellite markers from the endangered Puerto Rican boa (Chilabothrusinornatus) using paired-end Illumina shotgun sequencing

被引：0
作者：
R. Graham Reynolds
Alberto R. Puente-Rolón
Karen Kolodzaike
Tiara Butler-Smith
机构：
[1] University of Massachusetts Boston,Department of Biology
[2] Universidad Interamericana de Puerto Rico,Departamento de Ciencias y Tecnología
来源：
Conservation Genetics Resources | 2014年 / 6卷
关键词：
Boidae; Conservation; Endangered species; Microsatellites; Next generation DNA sequencing;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
We developed 23 microsatellite loci for the endangered Puerto Rican boa, Chilabothrusinornatus, from 100 bp reads obtained from a paired-end Illumina shotgun library. Importantly, these loci can be amplified using the same touchdown PCR program. All loci were highly polymorphic, with between 5 and 15 alleles found among 24 individuals genotyped from three populations. We anticipate that these loci will be extremely useful in conservation genetic studies of this species.
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页码：107 / 109
页数：2
相关论文
共 22 条
  • [1] Castoe TA(2012)Rapid microsatellite identification from Illumina paired-end genomic sequencing in two birds and a snake PLoS ONE 7 e30953-295
  • [2] Poole AW(2006)Genalex 6: genetic analysis in Excel. Population genetic software for teaching and research Mol Ecol Notes 6 288-249
  • [3] Jason de Koning AP(2013)Preliminary genetic analysis supports cave populations as targets for conservation in the endemic, endangered Puerto Rican boa (Boidae: PLoS ONE 8 e63899-538
  • [4] Jones KL(1995)) J Hered 86 248-undefined
  • [5] Tomback DF(2004)GenePop v1.2: population genetics software for exact test and ecumenicism Mol Ecol Notes 4 535-undefined
  • [6] Oyler-McCance SJ(undefined)MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data undefined undefined undefined-undefined
  • [7] Fike JA(undefined)undefined undefined undefined undefined-undefined
  • [8] Lance SL(undefined)undefined undefined undefined undefined-undefined
  • [9] Streicher JW(undefined)undefined undefined undefined undefined-undefined
  • [10] Smith EN(undefined)undefined undefined undefined undefined-undefined
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