Neurotrophin Expressions in Neural Stem Cells Grafted Acutely to Transected Spinal Cord of Adult Rats Linked to Functional Improvement

被引：0

作者：

Ying-Li Gu

Lu-Wei Yin

Zhuo Zhang

Jia Liu

Su-Juan Liu

Lian-Feng Zhang

Ting-Hua Wang

机构：

[1] Sichuan University,Department of Neurology, West China Hospital

[2] Institute of Neuroscience,Institute of Laboratory Animal Science

[3] Kunming Medical College,Translational Neuroscience Center

[4] CAMS,Department of Neurology

[5] Institute of Neurological Disease,undefined

[6] West China Hospital,undefined

[7] Sichuan University,undefined

[8] Tangdu Hospital,undefined

[9] Fourth Military Medical University,undefined

来源：

Cellular and Molecular Neurobiology | 2012年 / 32卷

关键词：

NSC; Transplantation; Sensory and locomotor functions; Rat;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

It is well known that neural stem cells (NSC) could promote the repairment after spinal cord injury, but the underlying mechanism remains to be elucidated. This study showed that the transplantation of NSC significantly improved hindlimb locomotor functions in adult rats subjected to transection of the spinal cord. Biotin dextran amine tracing together with the stimulus experiment in motor sensory area showed that little CST regeneration existed and functional synaptic formation in the injury site. Immunocytochemistry and RT-PCR demonstrated the secretion of NGF, BDNF, and NT-3 by NSC in vitro and in vivo, respectively. However, only mRNA expression of BDNF and NT-3 but not NGF in injury segment following NSC transplantation was upregulated remarkably, while caspase-3, a crucial apoptosis gene, was downregulated simultaneously. These provided us a clue that the functional recovery was correlated with the regulation of BDNF, NT-3, and caspase-3 in spinal cord transected rats following NSC transplantation.

引用

页码：1089 / 1097

页数：8

共 188 条

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