3D nitrogen-doped graphite foam@Prussian blue: an electrochemical sensing platform for highly sensitive determination of H2O2 and glucose

被引:0
作者
Yu Zhang
Bintong Huang
Feng Yu
Qunhui Yuan
Meng Gu
Junyi Ji
Yang Zhang
Yingchun Li
机构
[1] Shihezi University,Key Laboratory of Xinjiang Plant Resources and Utilization, Ministry of Education, School of Pharmacy
[2] Shihezi University,Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering
[3] Harbin Institute of Technology,School of Materials Science and Engineering
[4] Southern University of Science and Technology,Department of Materials Science and Engineering
[5] Sichuan University,Collage of Chemical Engineering
[6] Harbin Institute of Technology (Shenzhen),Shenzhen Key Laboratory of Organic Pollution Prevention and Control
[7] Harbin Institute of Technology,College of Science
来源
Microchimica Acta | 2018年 / 185卷
关键词
Biosensor; Nitrogen doped graphene foam; Glucose oxidase; Hydrogen peroxide; Dielectric barrier discharge plasma; Prussian blue; Amperometry; Scanning electron microscopy; X-ray photoelectron spectroscopy; Raman spectra;
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摘要
A platform is described for voltammetric sensing of hydrogen peroxide (H2O2). It is based on the use of nitrogen-doped graphite foam modified with Prussian Blue particles (PB/NGF). Graphite foam was synthesized by chemical vapor deposition, and doping with nitrogen was realized via dielectric barrier plasma discharge. PB particles were grown on the NGF through electrodeposition. SEM images of NGF verified the porous and interconnected structure of graphite foam, and XPS and Raman spectroscopy verified the successful doping with N. The performance of the PB/NGF electrode was characterized by CV and EIS which showed it to possess outstanding properties in terms of sensing H2O2. H2O2 was quantified in a range of 0.004 to 1.6 mM with a detection limit of 2.4 μM. The PB/NGF electrode also is shown to be a viable substrate for loading glucose oxidase (GOx). The GOx-functionalized electrode responds to glucose over the 0.2 to 20 mM concentration range at a potential of −50 mV (vs. Ag/AgCl), with a sensitivity of 27.48 mA M−1 cm−2 and a 0.1 M detection limit (at an S/N ratio of 3). The glucose sensor is selective, stable, and reproducible. The biosensor was successfully applied to the determination of glucose in spiked human serum samples, and this confirmed it practicability.
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