The extremely high level expression of human serum albumin in the milk of transgenic mice

被引:0
作者
Xiaojie Wu
Yanli Lin
Fuyin Xiong
Yanrong Zhou
Fang Yu
Jixian Deng
Peitang Huang
Hongxing Chen
机构
[1] Beijing Institute of Biotechnology,Cell Engineering Department
来源
Transgenic Research | 2012年 / 21卷
关键词
Whey acidic protein; Serum albumin; Milk; Transgenic mice;
D O I
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中图分类号
学科分类号
摘要
The recombinant production of human serum albumin has been challenging due to the low unit price and huge amount needed, for the commercial production of rhSA at an economically feasible level, It will be well worth the effort to exploit new method for the extremely high level expression of rhSA. To this end, here a hybrid gene locus strategy was employed, a 37 Kb mWAP-hSA hybrid gene locus was constructed and used as mammary gland specific expression vector, in which the 3 Kb genomic coding sequence in the 24 Kb mouse whey acidic protein (mWAP) gene locus was substituted by the 16 Kb genomic coding sequence of human serum albumin (hSA), exactly from the start codon to the end codon. Corresponding transgenic mice were generated and rhSA was secreted into the milk at an extremely high level of 11.9 g/L. Our transgenic mice carrying the mWAP-hSA hybrid gene locus represent a model system for the cost-effective production of human serum albumin.
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页码:1359 / 1366
页数:7
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