Adenovirus-mediated high expression of BCL-6 in CV-1 cells induces apoptotic cell death accompanied by down-regulation of BCL-2 and BCL-XL

被引：0
作者：
Tadanori Yamochi
Yoshitaka Kaneita
Tetsu Akiyama
Shigeo Mori
Masatsugu Moriyama
机构：
[1] the Institute of Medical Science,Department of Pathology
[2] the University of Tokyo,Department of Oncogene Research
[3] 4-6-1,undefined
[4] Research Institute for Microbial Diseases,undefined
[5] Osaka University,undefined
来源：
Oncogene | 1999年 / 18卷
关键词：
BCL-6; apoptosis; cell cycle; BCL-2; recombinant adenovirus;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The BCL-6 proto-oncogene encodes a 92- to 98-kDa transcriptional repressor containing the BTB/POZ domain at its N-terminal region and the zinc finger domain at its C-terminal region, respectively. In the present study, we examined the function of BCL-6 by using a recombinant adenovirus expressing BCL-6 (Ax1CA-BCL-6) and the lacZ reporter gene (Ax1CA-lacZ). Viability of CV-1 and HeLa cells infected with Ax1CA-BCL-6 was markedly reduced due to apoptosis, suggesting that BCL-6-overexpression induces apoptosis in CV-1 and HeLa cells. FACS analysis revealed that BCL-6-overexpressing cells are accumulated not only at the sub-G1 but also at G2/M phase. Induction of apoptosis by BCL-6 was preceded by down-regulation of apoptosis repressors BCL-2 and BCL-XL. These results suggest that BCL-6 induces apoptosis by regulating the expression of these apoptosis-regulating genes.
引用
收藏
页码：487 / 494
页数：7
相关论文
共 43 条
[1]  
Choi Michael S. K.(1995)The role of bcl-xL in CD40-mediated rescue from anti-μ-induced apoptosis in WEHI-231 B lymphoma cells European Journal of Immunology 25 1352-1357
[2]  
Boise Lawrence H.(1992)Features of apoptotic cells measured by flow cytometry Cytometry 13 795-808
[3]  
Gottschalk Alexander R.(1997)Control of Inflammation, Cytokine Expression, and Germinal Center Formation by BCL-6 Science 276 589-592
[4]  
Quintans José(1991)Germinal center cells express bcl-2 protein after activation by signals which prevent their entry into apoptosis European Journal of Immunology 21 1905-1910
[5]  
Thompson Craig B.(1997)BCL-6 is phosphorylated at multiple sites in its serine- and proline-clustered region by mitogen-activated protein kinase (MAPK) in vivo Oncogene 14 2465-2474
[6]  
Klaus Gerry G. B.(1991)Efficient selection for high-expression transfectants with a novel eukaryotic vector Gene 108 193-199
[7]  
Darzynkiewicz Z.(1993)Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programed cell death Cell 74 609-619
[8]  
Bruno S.(1993)B-cell apoptosis induced by antigen receptor crosslinking is blocked by a T-cell signal through CD40 Nature 364 645-648
[9]  
Del Bino G.(1993)Alterations of a zinc finger-encoding gene, BCL-6, in diffuse large-cell lymphoma Science 262 747-750
[10]  
Gorczyca W.(undefined)undefined undefined undefined undefined-undefined
12345