Chitinase Production in Solid-State Fermentation by Enterobacter sp. NRG4 Using Statistical Experimental Design

被引:0
作者
Neetu Dahiya
Rupinder Tewari
Ram Prakash Tiwari
Gurinder Singh Hoondal
机构
[1] Panjab University,Department of Biotechnology
[2] Panjab University,Department of Microbiology
来源
Current Microbiology | 2005年 / 51卷
关键词
Chitin; Response Surface Methodology; Chitinase; Wheat Bran; Central Composite Design;
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学科分类号
摘要
The optimization of nutrient levels for chitinase production by Enterobacter sp. NRG4 in solid-state fermentation conditions (SSF) was carried out using response surface methodology (RSM) based on central composite design (CCD). The design was employed by selecting wheat bran-to-flake chitin ratio, moisture level, inoculum size, and incubation time as model factors. The results of first-order factorial design experiments showed that all four independent variables have significant effects on chitinase production. The optimum concentrations for chitinase production were wheat bran-to-flake chitin ratio, 1; moisture level, 80%; inoculum size, 2.6 mL; and incubation time, 168 h. Using this statistical optimization method, chitinase production was found to increase from 616 U · g−1 dry weight of solid substrate to 1475 U · g−1 dry weight of solid substrate.
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页码:222 / 228
页数:6
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