Kineococcus glutineturens sp. nov., isolated from soil in Yunnan, south-west China

An orange-coloured, non-spore-forming, motile and coccus-shaped actinobacterium, designated YIM 75677T, was isolated from a soil sample collected from a dry-hot river valley in Dongchuan county, Yunnan Province, south-west China and its taxonomic position was investigated. Growth of strain YIM 75677T occurred at 12–55 °C, pH 6.0–9.0 and NaCl tolerance up to 2 % (w/v). Cells adhered to agar media and were agglutinated tightly together. The peptidoglycan contained meso-diaminopimelic acid, alanine and glutamic acid. The whole-cell hydrolysates mainly contained glucose, galactose, mannose and ribose. The predominant menaquinone was MK-9 (H2) and the major fatty acids were anteiso-C15:0 and iso-C15:0. Mycolic acids were not present. The DNA G+C content of strain YIM 75677T was 74.8 mol%. Phylogenetic analyses based on 16S rRNA gene sequence comparisons clearly revealed that strain YIM 75677T represents a novel member of the genus Kineococcus and is closely related to Kineococcus xinjiangensis S2-20T (level of similarity, 98.6 %). Meanwhile, the result of DNA–DNA hybridization between strain YIM 75677T and K. xinjiangensis S2-20T demonstrated that this isolate represented a different genomic species in the genus Kineococcus. On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, strain YIM 75677T represents a novel species of the genus Kineococcus, for which the name Kineococcusglutineturens sp. nov. is proposed. The type strain is YIM 75677T (=CCTCC AA 209075T = JCM 18126T).