Brassinolide enhances glucosinolates biosynthesis in shoot culture of Chinese cabbage (Brassica rapa ssp. pekinensis)

被引：0
作者：
Ganeshan Sivanandhan
Jiae Moon
Chaemin Sung
Solhee Bae
Chetan Kaur
Natesan Selvaraj
Yong Pyo Lim
机构：
[1] Chungnam National University,Department of Horticulture, College of Agriculture and Life Science
[2] Department of Botany,undefined
来源：
Plant Cell, Tissue and Organ Culture (PCTOC) | 2024年 / 157卷
关键词：
Elicitation; Secondary metabolites; Glucosinolates, shoot culture, Anti-oxidant; Exposure time; Transcription factor; Gene expression;
D O I：
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学科分类号：
摘要：
Chinese cabbage (Brassica rapa ssp. pekinensis), a green vegetable with high nutritional value, has been found to be rich in glucosinolates. Brassinosteroids (BRs), which regulate various plant growth and development processes, have been studied for their effect on glucosinolate production in in vitro shoot cultures. In this study, the most active compound in brassinosteroids, brassinolide, was used at different concentrations to test its effect on glucosinolate biosynthesis. A total glucosinolate content of 8.512 µmol g−1 DW was observed on MS optimal medium supplemented with 0.2 mg L−1 brassinolide. The expression of key genes related to glucosinolate biosynthesis and brassinolide signaling pathways was upregulated by brassinolide treatment. This research provides valuable insights for pharmaceutical and food companies interested in producing consistent levels of glucosinolates in Chinese cabbage shoots through the use of brassinolide.
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[1]  
Aires A(2016)Induced production of indol-3-ylmethyl glucosinolates in hairy roots of Chinese cabbage (Brassica rapa subsp. pekinensis): perspectives to enhance the content of bioactive compounds III Int Symp Hortic Eur-SHE 1242 49-56
[2]  
Neumann M(2020)Liquiritin elicitation can increase the content of medicinally important glucosinolates and phenolic compounds in Chinese kale plants J Sci Food Agric 100 1616-1624
[3]  
Ludwig-Müller J(2015)Comparison of glucosinolate profiles in different tissues of nine Brassica crops Molecules 20 15827-15841
[4]  
Schöpe M(2008)Glucosinolates in Brassica foods: bioavailability in food and significance for human health Phytochem Rev 7 213-229
[5]  
Van Pee KH(2018)Production of bioactive compounds and gene expression alterations in hairy root cultures of Chinese cabbage elicited by copper oxide nanoparticles Plant Cell Tissue Organ Cult 134 95-106
[6]  
Akram W(2006)Genetic and environmental variation of glucosinolate content in Chinese cabbage HortScience 41 1382-1385
[7]  
Saeed T(2018)Brassinosteroids regulate glucosinolate biosynthesis in Arabidopsis thaliana Physiol Plant 163 450-458
[8]  
Ahmad A(2001)Analysis of relative gene expression data using real-time quantitative PCR and the 2 Methods 25 402-408
[9]  
Yasin NA(1962) method Physiol Plant 15 473-497
[10]  
Akbar M(2014)A revised medium for rapid growth and bioassays with tobacco tissue cultures Food Chem 145 77-85
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