Excipients activate peroxidases in specific but not in non-specific reactions in organic solvents

被引：0
作者：
Xie Yuchun
Prasanta Kumar Das
Alexander M. Klibanov
机构：
[1] Massachusetts Institute of Technology,Department of Chemistry
[2] Cambridge,undefined
来源：
Biotechnology Letters | 2001年 / 23卷
关键词：
expoxidations; lyophilization; nonaqueous biocatalysis; peroxidase; sulfoxidations;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Co-lyophilization with ligands and lyoprotectants markedly activated horseradish and soybean peroxidases in the oxidation of guaiacol in organic solvents (Dai & Klibanov (1999) Proc. Natl. Acad. Sci. USA96: 9475–9478). However, such excipients gave little or no activation of these enzymes in non-specific reactions, sulfoxidation of thioanisole and epoxidation of styrene, presumably because they do not require a precise fine tuning of the enzyme active site.
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页码：1451 / 1454
页数：3
相关论文
共 33 条
[1]  
Adam W(1999)Biotransformations with peroxidases Adv. Biochem. Eng./Biotechnol. 63 73-108
[2]  
Lazarus M(1999)Recent biotechnological developments in the use of peroxidases Trends Biotechnol. 17 163-168
[3]  
Saha-Möller CR(1999)Striking activation of oxidative enzymes suspended in nonaqueous media Proc. Natl. Acad. Sci. USA 96 9475-9478
[4]  
Weichold O(2000)Peroxidase-catalyzed asymmetric sulfoxidation in organic solvents versus in water Biotechnol. Bioeng. 70 353-357
[5]  
Hoch U(1987)Polymerization of phenols catalyzed by peroxidase in non-aqueous media Biotechnol. Bioeng. 30 31-36
[6]  
Häring D(1995)Lyophilization-induced changes in the secondary structure of proteins Proc. Natl. Acad. Sci. USA 92 10969-10976
[7]  
Schreier P(1993)Horseradish peroxidase-catalyzed 2-electron oxidations-oxidation of iodide, thioanisole, and phenols at distinct sites J. Biol. Chem. 268 1637-1645
[8]  
Colonna S(1997)Why are enzymes less active in organic solvents than in water? Trends Biotechnol. 15 97-101
[9]  
Gaggero N(2001)Improving enzymes by using them in organic solvents Nature 409 241-246
[10]  
Richelmi C(1997)Conversion of myoglobin into a peroxygenase: a catalytic intermediate of sulfoxidation and epoxidation by the F43H/H64L mutant J. Am. Chem. Soc. 117 7056-7064
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