Isolation and Characterization of Twenty Microsatellite Loci in Japanese Sea Cucumber (Stichopus japonicus)

被引：0
作者：
Manami Kanno
Qi Li
Akihiro Kijima
机构：
[1] Tohoku University,Integrated Center of Field Sciences, Graduate School of Agricultural Science
[2] Ocean University of China,Fisheries College
来源：
Marine Biotechnology | 2005年 / 7卷
关键词：
sea cucumber; microsatellite loci; genetic variability;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Twenty microsatellite markers were first developed from the Japanese sea cucumber Stichopus japonicus using an enrichment protocol. Of the 20 microsatellite loci, 19 loci were polymorphic in the population examined. At these polymorphic loci, the number of alleles per locus varied from 2 to 15, and the observed heterozygosities ranged from 0.03 to 0.97, which is considerably higher than those previously found for allozymes. The high variability of the microsatellite markers identified in this study will make them excellent tools for genetic analyses of S. japonicus.
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页码：179 / 183
页数：4
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